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dc.rights.licenseopenen_US
hal.structure.identifierLeibniz-Institut für Polymerforschung Dresden e.V. [IPF]
dc.contributor.authorMORENO, Silvia
hal.structure.identifierLeibniz-Institut für Polymerforschung Dresden e.V. [IPF]
dc.contributor.authorBOYE, Susanne
hal.structure.identifierLeibniz-Institut für Polymerforschung Dresden e.V. [IPF]
dc.contributor.authorLEDERER, Albena
hal.structure.identifierUniversity of Naples Federico II = Università degli studi di Napoli Federico II
dc.contributor.authorFALANGA, Annarita
hal.structure.identifierUniversity of Naples Federico II = Università degli studi di Napoli Federico II
dc.contributor.authorGALDIERO, Stefania
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
hal.structure.identifierLeibniz-Institut für Polymerforschung Dresden e.V. [IPF]
hal.structure.identifierCenter for Advancing Electronics in Dresden [CFAED]
dc.contributor.authorVOIT, Brigitte
hal.structure.identifierLeibniz-Institut für Polymerforschung Dresden e.V. [IPF]
dc.contributor.authorAPPELHANS, Dietmar
dc.date.accessioned2020-12-24T09:21:31Z
dc.date.available2020-12-24T09:21:31Z
dc.date.issued2020-11-12
dc.identifier.issn1525-7797en_US
dc.identifier.urioai:crossref.org:10.1021/acs.biomac.0c01276
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/23597
dc.description.abstractEnTo mimic organelles and cells and to construct next-generation therapeutics, asymmetric functionalization and location of proteins for artificial vesicles is thoroughly needed to emphasize the complex interplay of biological units and systems through spatially separated and spatiotemporal controlled actions, release, and communications. For the challenge of vesicle (= polymersome) construction, the membrane permeability and the location of the cargo are important key characteristics that determine their potential applications. Herein, an in situ and post loading process of avidin in pH-responsive and photo-cross-linked polymersomes is developed and characterized. First, loading efficiency, main location (inside, lumen, outside), and release of avidin under different conditions have been validated, including the pH-stable presence of avidin in polymersomes’ membrane outside and inside. This advantageous approach allows us to selectively functionalize the outer and inner membranes as well as the lumen with several bio(macro)molecules, generally suited for the construction of asymmetrically functionalized artificial organelles. In addition, a fluorescence resonance energy transfer (FRET) effect was used to study the permeability or uptake of the polymersome membrane against a broad range of biotinylated (macro)molecules (different typology, sizes, and shapes) under different conditions.
dc.language.isoENen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.sourcecrossref
dc.subject.enPurification
dc.subject.enMacromolecules
dc.subject.enMembranes
dc.subject.enPermeability
dc.subject.enFluorescence resonance energy transfer
dc.title.enAvidin Localizations in pH-Responsive Polymersomes for Probing the Docking of Biotinylated (Macro)molecules in the Membrane and Lumen
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/acs.biomac.0c01276en_US
dc.subject.halChimie/Polymères
dc.identifier.pubmed33180486en_US
bordeaux.journalBiomacromoleculesen_US
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-03089668
hal.version1
hal.date.transferred2020-12-28T16:22:41Z
hal.exporttrue
workflow.import.sourcedissemin
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