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Coupling of RAFT polymerization and chemoselective post-modifications of elastin-like polypeptides for the synthesis of gene delivery hybrid vectors

hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorBRAVO ANAYA, Lourdes Monica M
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
hal.structure.identifierTeam 2 LCPO : Biopolymers & Bio-sourced Polymers
dc.contributor.authorROSSELGONG, Julien
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorFERNÁNDEZ-SOLÍS, Karla Gricelda
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorXIAO, Ye
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorVAX, Amélie
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorIBARBOURE, Emmanuel
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorRUBAN, Anna
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLEBLEU, Coralie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierBiotechnologie des protéines recombinantes à visée santé
dc.contributor.authorJOUCLA, Gilles
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGARBAY, Bertrand
ORCID: 0000-0001-5756-2627
IDREF: 033777551
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGARANGER, Elisabeth
IDREF: 089451740
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
dc.date.accessioned2020-12-01T14:40:19Z
dc.date.available2020-12-01T14:40:19Z
dc.date.issued2020-11-21
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/21282
dc.description.abstractEnHybrids of synthetic polymers and biopolymers are known to be macromolecular systems that merge the properties of each component and overcome some of their intrinsic limitations. Elastin-like polypeptides (ELPs) are a class of biopolymers known for their genetically-encoded synthesis, monodispersity, biocompatibility and absence of toxicity, which are very attractive features for biological applications. However, the presence of numerous amino acid residues presenting electrically charged side chains within the ELP sequence makes the purification of these ELPs by Inverse Transition Cycling(ITC) difficult to achieve. Positively charged ELPs are of great interest for the design of polyelectrolyte complexes dedicated to the transport and delivery of genetic material. In this work, an ELP containing periodically spaced methionine residues was recombinantly expressed, and these residues were chemoselectively modified at the thioether side chains to introduce alkyne groups. In parallel, four cationic oligomers with different chain lengths were synthesized by Reversible Addition-Fragmentation chain Transfer (RAFT) polymerization using a chain transfer agent containing an azido group. Hybrid cationic ELP were finally obtained by covalent coupling of the cationic oligomers onto the ELP by Huisgen azide-alkyne cycloaddition reaction. The different hybrid cationic ELPs were characterized by 1H NMR, ζ-potential and SEC analyses to assess their purity and determine their degree of functionalization, overall charge and molar mass. Then, electrostatic complexation was achieved between these hybrid cationic ELPs and plasmid DNA, allowing the determination of the optimal conditions for obtaining stable nanoparticles having a controlled size and surface potentials at different N+/P- charge ratios. Preliminary biological tests showed the reliability of such hybrid cationic ELPs to internalize efficiently genetic material into living cells
dc.title.enCoupling of RAFT polymerization and chemoselective post-modifications of elastin-like polypeptides for the synthesis of gene delivery hybrid vectors
dc.typeArticle de revueen_US
dc.identifier.doi10.1039/D0PY01293A
dc.subject.halChimie/Matériaux
bordeaux.journalPolymer Chemistryen_US
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629en_US
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
hal.identifierhal-03034155
hal.version1
hal.date.transferred2020-12-01T15:20:02Z
hal.exporttrue
workflow.import.sourcepubmed
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