Afficher la notice abrégée

Aquarius helicase facilitates HIV-1 integration into R-loop enriched genomic regions.

dc.rights.licenseopenen_US
dc.contributor.authorPENZO, Carlotta
dc.contributor.authorÖZEL, Ilayda
dc.contributor.authorMARTINOVIC, Moreno
dc.contributor.authorKUZMAN, Maja
dc.contributor.authorGLAVAS, Dunja
dc.contributor.authorSTANIC, Mia
dc.contributor.authorREICHENBACH, Thomas
dc.contributor.authorMÜLLER, Thorsten G
dc.contributor.authorRHEINBERGER, Mona
dc.contributor.authorGODARZI, Negar
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAPAILLERIE, Delphine
dc.contributor.authorSREZOVIC, Bruno
dc.contributor.authorDELL'OCA, Maria Chiara
dc.contributor.authorLANGE, Laura C
dc.contributor.authorSADHU, Lopamudra
dc.contributor.authorDE CASTRO, Ines J
dc.contributor.authorSHYTAJ, Iart Luca
dc.contributor.authorFORCATO, Mattia
dc.contributor.authorLAKETA, Vibor
dc.contributor.authorBICCIATO, Silvio
dc.contributor.authorVLAHOVICEK, Kristian
dc.contributor.authorFACKLER, Oliver T
dc.contributor.authorLUCIC, Bojana
dc.contributor.authorPENA, Vlad
dc.contributor.authorKRÄUSSLICH, Hans-Georg
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.contributor.authorLUSIC, Marina
dc.date.accessioned2025-09-01T08:31:07Z
dc.date.available2025-09-01T08:31:07Z
dc.date.issued2025-08-20
dc.identifier.issn2058-5276en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207546
dc.description.abstractEnHIV-1 integration into host chromosomes, essential for viral replication, is catalysed by viral integrase (IN). IN recurrently targets intronic regions of transcriptionally active genes, but a detailed understanding of this process is still unclear. Here, using ex vivo activated human primary CD4T cells, we find that genomic RNA:DNA hybrids (R-loops) preferentially map to intronic regions of active genes that are typical HIV-1 integration sites. IN binds R-loops and their resolution enhances viral integration in vitro. We identify Aquarius (AQR), the splicing RNA helicase of the pentameric intron binding complex (IBC), which associates with IN and show that its RNA:DNA helicase activity promotes integration into hybrid substrates in vitro. Knockout of AQR in primary CD4 T cells impaired overall integration efficiency, while sequencing of remaining integrations mapped them to intergenic and R-loop distal regions. These findings may have important implications for HIV-1 latency and reactivation and may thus identify novel therapeutic targets.
dc.language.isoENen_US
dc.title.enAquarius helicase facilitates HIV-1 integration into R-loop enriched genomic regions.
dc.title.alternativeNat Microbiolen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41564-025-02089-2en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed40836041en_US
bordeaux.journalNature Microbiologyen_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-05232588
hal.version1
hal.date.transferred2025-09-01T08:31:12Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Microbiology&rft.date=2025-08-20&rft.eissn=2058-5276&rft.issn=2058-5276&rft.au=PENZO,%20Carlotta&%C3%96ZEL,%20Ilayda&MARTINOVIC,%20Moreno&KUZMAN,%20Maja&GLAVAS,%20Dunja&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée