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Interaction with eukaryotic cells enhances the circularization and transcription of integrative and conjugative elements in Mycoplasma hominis.

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBOUREIMA ABDOU, Bachir
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorSILVANT, Alicia
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLE ROY, Chloé
dc.contributor.authorGUIRAUD, Jennifer
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBIENTZ, Léa
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉVEN, Laure
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorDUBOIS, Véronique
dc.contributor.authorBARANOWSKI, Eric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBÉBÉAR, Cécile
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPEREYRE, Sabine
dc.date.accessioned2025-07-17T10:25:42Z
dc.date.available2025-07-17T10:25:42Z
dc.date.issued2025-07-04
dc.identifier.issn1759-8753en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/207345
dc.description.abstractEnMobile genetic elements drive bacterial evolution by promoting genetic plasticity and adaptation; among them, mycoplasma Integrative and Conjugative Elements (ICEs) challenge the notion that mycoplasmas evolved solely through genome reduction. However, they remain poorly characterized, particularly in Mycoplasma hominis, which is a human genital pathogen. In this study, we investigated the circularization, transcription, and protein expression of M. hominis 4788 ICE (ICEHo4788) under the following four environmental conditions: axenic growth, coculture with HeLa cells, mitomycin C exposure, and thermal stress. During axenic growth, ICEHo4788 circularization peaked at 12 h with a 9.4-fold increase in the number of circular forms. Mitomycin C and cold shock induced only a moderate increase in circularization (approximately 3-fold), whereas heat shock significantly reduced the number of circular forms. In coculture with HeLa cells, a strong increase in the number of circular forms was observed at 72 h and 7 days postinfection with 10- and 23-fold increases, respectively. Coculture also led to a 5- to 23-fold increase in transcription at 7 days, whereas axenic growth, mitomycin C exposure, and thermal stress did not differ. Proteomic analysis revealed that MhoH(a), CDS18, CDS17, and CDS11 were significantly overexpressed at 12 h. Neither mitomycin C nor cold shock induced changes in ICE-related proteins, but heat shock upregulated two M. hominis molecular chaperones and ICEHo4788 MhoH(a). In conclusion, environmental conditions modulate M. hominis ICE activity with strong stimulation in the presence of eukaryotic cells. These findings offer new insights into the regulation and environmental responses of M. hominis ICEs.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enMycoplasma hominis; Circularization; HeLa cells; Integrative and conjugative element; Protein expression; Transcription
dc.title.enInteraction with eukaryotic cells enhances the circularization and transcription of integrative and conjugative elements in Mycoplasma hominis.
dc.title.alternativeMob DNAen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1186/s13100-025-00364-xen_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed40616119en_US
bordeaux.journalMobile DNAen_US
bordeaux.page26en_US
bordeaux.volume16en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue1en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-05167347
hal.version1
hal.date.transferred2025-07-17T10:25:45Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Mobile%20DNA&rft.date=2025-07-04&rft.volume=16&rft.issue=1&rft.spage=26&rft.epage=26&rft.eissn=1759-8753&rft.issn=1759-8753&rft.au=BOUREIMA%20ABDOU,%20Bachir&SILVANT,%20Alicia&LE%20ROY,%20Chlo%C3%A9&GUIRAUD,%20Jennifer&BIENTZ,%20L%C3%A9a&rft.genre=article


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