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Virome Scanning of Pear Germplasm Collections Identifies a New Velarivirus and Extends the Geographical Spread of Three Other Pear Viruses

hal.structure.identifierGembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
dc.contributor.authorFONTDEVILA PARETA, Nuria
hal.structure.identifierGembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
dc.contributor.authorGAILLY, Carole
hal.structure.identifierAgroscope
dc.contributor.authorBLOUIN, Arnaud
hal.structure.identifierAgroscope
dc.contributor.authorBUCHMANN, Beatrix
hal.structure.identifierAgroscope
dc.contributor.authorBÜNTER, Markus
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCANDRESSE, Thierry
hal.structure.identifierAgroscope
dc.contributor.authorDUBUIS, Nathalie
hal.structure.identifierNational Institute of Biology [Ljubljana] [NIB]
dc.contributor.authorKUTNJAK, Denis
hal.structure.identifierCentre wallon de Recherches Agronomiques [Belgique] = Walloon Agricultural Research Centre [Belgium] [CRA-W]
dc.contributor.authorLATEUR, Marc
hal.structure.identifierNational Institute of Biology [Ljubljana] [NIB]
dc.contributor.authorPECMAN, Anja
hal.structure.identifierCentre wallon de Recherches Agronomiques [Belgique] = Walloon Agricultural Research Centre [Belgium] [CRA-W]
dc.contributor.authorSTEYER, Stéphan
hal.structure.identifierGembloux Agro-Bio Tech [Faculté universitaire des sciences agronomiques de Gembloux] [[FUSAGx]]
dc.contributor.authorMASSART, Sébastien
dc.date.issued2025-03
dc.description.abstractEnIn this study, an extensive virome investigation was performed on a germplasm collection of pear trees ( Pyrus communis L.) from the Walloon Agricultural Research Centre (Gembloux, Belgium). In total, 128 pear trees samples were analyzed as pools using high-throughput sequencing techniques and/or tested individually for targeted viruses by reverse transcription PCR (RT-PCR). During the virome survey, a novel velarivirus was identified in several asymptomatic trees, and four known viruses were detected. Bioinformatics tools were used to assemble the genome of the new virus. The pear germplasm collection from Kozjanski Park (Slovenia) and a viral collection from Agroscope (Nyon, Switzerland) were also surveyed for the new pear virus and for three known viruses (citrus virus A [CiVA], apple rubbery wood virus 1 [ARWV-1], and apple rubbery wood virus 2 [ARWV-2]) to study their prevalence and geographic distribution. In Belgium, the new velarivirus was detected by RT-PCR in 6 of the 99 sampled trees (6%) and CiVA in 49 (49%) of them; in Slovenia, four of the six trees sampled (67%) were positive for CiVA; and in Switzerland, four of the nine trees sampled (44%) were positive for CiVA and 1 (11%) for ARWV-1 and -2. This study combined pooled high-throughput sequencing analyses to maximize the amount of germplasm tested and targeted RT-PCR tests on individual samples for accurate detection. It reports and describes a new velarivirus discovered in pear trees and first detections of CiVA in Belgium, Switzerland, and Slovenia, as well as ARWV-1 and -2 in Switzerland. [Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
dc.language.isoen
dc.publisherAPS Publications
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enapple rubbery wood virus 1
dc.subject.enapple rubbery wood virus 2
dc.subject.encitrus virus A
dc.subject.enhigh-throughput sequencing
dc.subject.enpear
dc.subject.enplant virus
dc.subject.enPyrus virus A
dc.subject.envelarivirus
dc.subject.envirome
dc.title.enVirome Scanning of Pear Germplasm Collections Identifies a New Velarivirus and Extends the Geographical Spread of Three Other Pear Viruses
dc.typeArticle de revue
dc.identifier.doi10.1094/PBIOMES-03-24-0030-FI
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPhytobiomes Journal
bordeaux.page68-79
bordeaux.volume9
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-05125733
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05125733v1
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