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LIPID DROPLETS: NEW ACTORS OF THE PLANT VIRUS INFECTION

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBATSALE, Marguerite
hal.structure.identifierNutrition et Neurobiologie intégrée [NutriNeuro]
dc.contributor.authorMARISSAL-ARVY, Nathalie
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorJAMBOU, Lena
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSOFER, Luc
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorSIMON, Vincent
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorQUINTEAU, Charlotte
hal.structure.identifierUniversity of Guelph [Guelf, Ontario, Canada]
dc.contributor.authorDONER, Nathan
hal.structure.identifierUniversity of Guelph [Guelf, Ontario, Canada]
dc.contributor.authorMULLEN, Robert
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorCOULON, Denis
hal.structure.identifierLaboratoire de biogenèse membranaire [LBM]
dc.contributor.authorBRÉHÉLIN, Claire
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGERMAN-RETANA, Sylvie
dc.date.accessioned2025-06-17T02:05:44Z
dc.date.available2025-06-17T02:05:44Z
dc.date.conference2025-04-08
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/206931
dc.description.abstractEnOver the past decade, there has been an increase in evidence that human positive single stranded RNA (+ssRNA) viruses extensively manipulate the host's lipid metabolism and use lipid droplets (LDs) to enhance energy production and promote viral replication and assembly (Monson et al., 2021). LDs are universal dynamic organelles with a core of neutral lipids surrounded by a phospholipid monolayer associated with enzymes and structural proteins. More than simple storage units, these organelles are key regulators of cellular homeostasis, playing significant roles in lipid metabolism, energy storage, and signal transduction (Bouchnak et al., 2023). Very recent studies have highlighted the potential role of LDs in plant responses to infections caused by both plant and fungal viruses (Dai et al., 2024; Wang et al., 2024). However, the mechanisms involving LDs in plant-virus interactions remain unexplored.In this study, we present the first comprehensive analysis of LD accumulation and their recruitment to viral replication compartments (VRCs) during infection by turnip mosaic virus (TuMV, a member of the Potyvirus genus) in Arabidopsis thaliana and Nicotiana benthamiana, using confocal microscopy. We also evidenced a significant accumulation of neutral lipids in infected leaves thus confirming that TuMV-infection induces LD biogenesis. In addition, Transmission Electron Microscopy data revealed that the newly formed LDs are located near TuMV-induced VRCs. In agreement with these results, our mRNA translatome analysis of TuMV-infected A. thaliana leaves revealed an increased translation of LD structural proteins, LIPID DROPLET ASSOCIATED PROTEINs (LDAPs), a family of proteins crucial for LD biosynthesis and stability, and recruitment of lipases. Moreover, TuMV propagation was significantly impaired in Arabidopsis ldap knock-out mutants and enhanced in LDAP overexpressing plants. Our preliminary data suggest that LDAP proteins may play roles in both viral movement and replication and therefore represent key factors in LD recruitment during viral infection. Collectively, our results point to a pro-viral function of LDs in TuMV-infected plants.
dc.description.sponsorshipRôle des gouttelettes lipidiques dans l'infection virale chez les plantes - ANR-23-CE20-0026
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enLIPID DROPLETS: NEW ACTORS OF THE PLANT VIRUS INFECTION
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.conference.titleInternational Advances in Plant Virology 2025
bordeaux.countryES
bordeaux.conference.cityMurcia
bordeaux.peerReviewednon
hal.identifierhal-05114255
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2025-04-11
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-05114255v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=BATSALE,%20Marguerite&MARISSAL-ARVY,%20Nathalie&JAMBOU,%20Lena&SOFER,%20Luc&SIMON,%20Vincent&rft.genre=unknown


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