Azole resistance in Aspergillus flavus and associated fitness cost
| dc.rights.license | open | en_US |
| dc.contributor.author | DJENONTIN, Elie | |
| hal.structure.identifier | Centre Hospitalier Régional Universitaire de Nancy [CHRU Nancy] | |
| dc.contributor.author | DEBOURGOGNE, Anne | |
| hal.structure.identifier | Dynamic Microbiology - EA 7380 [DYNAMIC] | |
| hal.structure.identifier | Mazandaran University of Medical Sciences | |
| dc.contributor.author | MOUSAVI, Bita | |
| hal.structure.identifier | Université de Bordeaux [UB] | |
| hal.structure.identifier | CHU de Bordeaux Pellegrin [Bordeaux] | |
| hal.structure.identifier | Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB] | |
| dc.contributor.author | DELHAES, Laurence | |
| hal.structure.identifier | Centre Hospitalier Universitaire [CHU Grenoble] [CHUGA] | |
| hal.structure.identifier | Translational microbial Evolution and Engineering [TIMC-TrEE] | |
| hal.structure.identifier | Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 [TIMC] | |
| hal.structure.identifier | Université Grenoble Alpes [UGA] | |
| dc.contributor.author | CORNET, Muriel | |
| hal.structure.identifier | Dynamic Microbiology - EA 7380 [DYNAMIC] | |
| dc.contributor.author | VALSECCHI, Isabel | |
| dc.contributor.author | ADEBO, Makiath | |
| hal.structure.identifier | École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique [ONIRIS] | |
| dc.contributor.author | GUILLOT, Jacques | |
| hal.structure.identifier | Hôpital Henri Mondor | |
| dc.contributor.author | BOTTEREL, Françoise | |
| hal.structure.identifier | Unité de Parasitologie-Mycologie, Service de Microbiologie [Hôpital Necker-Enfants-Malades, Paris] | |
| dc.contributor.author | DANNAOUI, Eric | |
| dc.date.accessioned | 2025-06-16T11:55:04Z | |
| dc.date.available | 2025-06-16T11:55:04Z | |
| dc.date.issued | 2024-07-15 | |
| dc.identifier.issn | 0933-7407 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/206920 | |
| dc.description.abstractEn | Abstract Background The resistance of Aspergillus flavus to the azole antifungal drugs is an emerging problem. Mutations in the molecular targets of the azole antifungals ‐ CYP 51 A, B and C ‐ are possible mechanisms of resistance, but data to confirm this hypothesis are scarce. In addition, the behaviour of resistant strains in vitro and in vivo is not yet understood. Objectives This study had 3 objectives. The first was to compare the sequences of CYP51 A, B and C in resistant and susceptible strains of A. flavus . The second was to look for the existence of a fitness cost associated with resistance. The third was to evaluate the activity of voriconazole and posaconazole on resistant strains in the Galleria mellonella model. Methods The CYP51 A, B and C sequences of seven resistant strains with those of four susceptible strains are compared. Fitness costs were assessed by growing the strains in RPMI medium and testing their virulence in G. mellonella larvae. In addition, G. mellonella larvae infected with strains of A. flavus were treated with voriconazole and posaconazole. Results In the CYP51A sequences, we found the A91T, C708T and A1296T nucleotide substitutions only in the resistant strains. The resistant strains showed a fitness cost with reduced in vitro growth and reduced virulence in G. mellonella . In vivo resistance to posaconazole is confirmed in a strain with the highest MIC for this antifungal agent. Conclusions These results allow to conclude that some substitutions in CYP51 genes, in particular CYP51A , contribute to resistance to azole drugs in A. flavus . The study of the relationship between drug dosage and treatment duration with resistance and the reduction of fitness costs in resistant strains is a major perspective of this study. This work could help to establish recommendations for the treatment of infections with resistant strains of A. flavus. | |
| dc.language.iso | EN | en_US |
| dc.title.en | Azole resistance in Aspergillus flavus and associated fitness cost | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1111/myc.13766 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biotechnologies | en_US |
| bordeaux.journal | Mycoses | en_US |
| bordeaux.page | 2169 | en_US |
| bordeaux.volume | 67 | en_US |
| bordeaux.hal.laboratories | Centre de Recherche Cardio-Thoracique de Bordeaux (CRCTB) - U1045 | en_US |
| bordeaux.issue | 7 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-04997696 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Mycoses&rft.date=2024-07-15&rft.volume=67&rft.issue=7&rft.spage=2169&rft.epage=2169&rft.eissn=0933-7407&rft.issn=0933-7407&rft.au=DJENONTIN,%20Elie&DEBOURGOGNE,%20Anne&MOUSAVI,%20Bita&DELHAES,%20Laurence&CORNET,%20Muriel&rft.genre=article |
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