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A novel in vitro tubular model to recapitulate features of distal airways: The bronchioid

dc.rights.licenseopenen_US
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
dc.contributor.authorMAURAT, Elise
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
dc.contributor.authorRAASCH, Katharina
hal.structure.identifierHelmholtz Centre for Infection Research [HZI]
dc.contributor.authorLEIPOLD, Alexander
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorHENROT, Pauline
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorZYSMAN, Maeva
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorPREVEL, Renaud
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
dc.contributor.authorTRIAN, Thomas
hal.structure.identifierHelmholtz Centre for Infection Research [HZI]
dc.contributor.authorKRAMMER, Tobias
hal.structure.identifierTBM-Core [Bordeaux] [UMS3427 - INSERM US005]
dc.contributor.authorBERGERON, Vanessa
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorTHUMEREL, Matthieu
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorNASSOY, Pierre
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
hal.structure.identifierCentre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
dc.contributor.authorBERGER, Patrick
ORCID: 0000-0003-4702-0343
IDREF: 060717998
hal.structure.identifierJulius-Maximilians-Universität Würzburg = University of Würzburg [Würsburg, Germany] [JMU]
hal.structure.identifierHelmholtz Centre for Infection Research [HZI]
dc.contributor.authorSALIBA, Antoine-Emmanuel
hal.structure.identifierTBM-Core [Bordeaux] [UMS3427 - INSERM US005]
dc.contributor.authorANDRIQUE, Laetitia
hal.structure.identifierLaboratoire Photonique, Numérique et Nanosciences [LP2N]
dc.contributor.authorRECHER, Gaëlle
hal.structure.identifierCentre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] [CRCTB]
hal.structure.identifierCIC Bordeaux
hal.structure.identifierInstitut universitaire de France [IUF]
dc.contributor.authorDUPIN, Isabelle
dc.date.accessioned2025-06-16T11:52:45Z
dc.date.available2025-06-16T11:52:45Z
dc.date.issued2024-09-04
dc.identifier.issn0903-1936en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/206919
dc.description.abstractEnBackground Airflow limitation is the hallmark of obstructive pulmonary diseases, with the distal airways representing a major site of obstruction. Although numerous in vitro models of bronchi already exist, there is currently no culture system for obstructive diseases that reproduces the architecture and function of small airways. Here, we aimed to engineer a model of distal airways to overcome the limitations of current culture systems. Methods We developed a so-called bronchioid model by encapsulating human bronchial adult stem cells derived from clinical samples in a tubular scaffold made of alginate gel. Results This template drives the spontaneous self-organisation of epithelial cells into a tubular structure. Fine control of the level of contraction is required to establish a model of the bronchiole, which has a physiologically relevant shape and size. 3D imaging, gene expression and single-cell RNA-seq analysis of bronchioids made of bronchial epithelial cells revealed tubular organisation, epithelial junction formation and differentiation into ciliated and goblet cells. Ciliary beating is observed, at a decreased frequency in bronchioids made of cells from COPD patients. The bronchioid can be infected by rhinovirus. An air-liquid interface is introduced that modulates gene expression. Conclusion Here, we provide a proof of concept of a perfusable bronchioid with proper mucociliary and contractile functions. The key advantages of our approach, such as the air‒liquid interface, lumen accessibility, recapitulation of pathological features and possible assessment of clinically relevant endpoints, will make our pulmonary organoid-like model a powerful tool for preclinical studies.
dc.language.isoENen_US
dc.rights.urihttp://hal.archives-ouvertes.fr/licences/copyright/
dc.title.enA novel in vitro tubular model to recapitulate features of distal airways: The bronchioid
dc.typeArticle de revueen_US
dc.identifier.doi10.1183/13993003.00562-2024en_US
dc.subject.halSciences du Vivant [q-bio]en_US
bordeaux.journalEuropean Respiratory Journalen_US
bordeaux.pageOnline ahead of printen_US
bordeaux.hal.laboratoriesCentre de Recherche Cardio-Thoracique de Bordeaux (CRCTB) - U1045en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-04741681
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=European%20Respiratory%20Journal&rft.date=2024-09-04&rft.spage=Online%20ahead%20of%20print&rft.epage=Online%20ahead%20of%20print&rft.eissn=0903-1936&rft.issn=0903-1936&rft.au=MAURAT,%20Elise&RAASCH,%20Katharina&LEIPOLD,%20Alexander&HENROT,%20Pauline&ZYSMAN,%20Maeva&rft.genre=article


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