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Biomonitoring of azole fungicides in free-living blackbird plasma using on-line solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry (SPE HPLC-MS/MS)

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorPROUTEAU, Louise
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDEVIER, Marie-Helene
IDREF: 072746092
dc.contributor.authorANGELIER, Frédéric
dc.contributor.authorCHASTEL, Olivier
dc.contributor.authorBRISCHOUX, François
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorPARDON, Patrick
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorLE MENACH, Karyn
IDREF: 256879966
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBUDZINSKI, Hélène
ORCID: 0000-0003-1028-9154
IDREF: 070478090
dc.date.accessioned2025-04-16T07:40:52Z
dc.date.available2025-04-16T07:40:52Z
dc.date.issued2025-05-10
dc.identifier.issn0021-9673en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/206218
dc.description.abstractEnIn this study, a rapid and sensitive method using on-line solid-phase extraction (SPE) coupled to liquid chromatography - tandem mass spectrometry (SPE HPLC-MS/MS) was developed to analyse 15 azole fungicides currently used in vineyards in blackbird plasma samples. The monitored fungicides included 13 triazoles (cyproconazole, difenoconazole, epoxiconazole, fenbuconazole, flusilazole, flutriafol, metconazole, penconazole, propiconazole, tebuconazole, tetraconazole, triadimefon, triadimenol) and 2 imidazoles (imazalil and prochloraz). After a rapid preparation step by protein precipitation with acetonitrile on 25 µL of plasma samples, final extracts diluted with Milli-Q water were analyzed by on-line SPE-LC-MS/MS in positive electrospray mode (ESI+) using the dynamic multi-reaction monitoring mode (dMRM). Following optimization, method validation was achieved through studies of linearity, sensitivity, accuracy, precision, and sample extract conservation. The limits of quantification (LOQs) obtained for a low volume of plasma (25 µL) ranged from 0.01 to 0.43 ng g−1 plasma, except for triadimenol (1.37 ng g−1). Finally, the validated method was successfully applied to 34 Eurasian blackbird plasma samples, with blackbirds from different habitats (city, forest, vineyards) submitted to contrasted azole pressures. Five of them were detected, tebuconazole and tetraconazole being the predominant ones. As expected, azoles concentrations were more elevated in blackbirds sampled in vineyards where most of these fungicides are used.
dc.language.isoENen_US
dc.title.enBiomonitoring of azole fungicides in free-living blackbird plasma using on-line solid-phase extraction coupled to liquid chromatography-tandem mass spectrometry (SPE HPLC-MS/MS)
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.chroma.2025.465725en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalJournal of Chromatography Aen_US
bordeaux.page465725en_US
bordeaux.volume1748en_US
bordeaux.hal.laboratoriesEPOCen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamLPTCen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcecrossref
hal.identifierhal-05036008
hal.version1
hal.date.transferred2025-04-16T07:40:55Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcecrossref
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Chromatography%20A&rft.date=2025-05-10&rft.volume=1748&rft.spage=465725&rft.epage=465725&rft.eissn=0021-9673&rft.issn=0021-9673&rft.au=PROUTEAU,%20Louise&DEVIER,%20Marie-Helene&ANGELIER,%20Fr%C3%A9d%C3%A9ric&CHASTEL,%20Olivier&BRISCHOUX,%20Fran%C3%A7ois&rft.genre=article


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