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Imaging xylem connections between the scion and rootstock of grafted grapevine with X-ray microtomography

dc.rights.licenseopenen_US
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorJANOUEIX, Anne
dc.contributor.authorSPILMONT, A.-S.
dc.contributor.authorMOISY, C.
dc.contributor.authorMATHIEU, G.
dc.contributor.authorCORDELIERES, F.
dc.contributor.authorTEILLON, J.
dc.contributor.authorGONZAGA SANTESTEBAN, Luis
dc.contributor.authorVILLA-LLOP, A.
dc.contributor.authorCRESPO, S.
dc.contributor.authorMARIN, D.
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorCOOKSON, Sarah Jane
IDREF: 22161009X
dc.contributor.editorBurger, J.T.
dc.contributor.editorVivier, M.A.
dc.date.accessioned2025-04-08T13:54:12Z
dc.date.available2025-04-08T13:54:12Z
dc.date.issued2024-03
dc.date.conference2021-10-31
dc.identifier.issn0567-7572en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/206042
dc.description.abstractEnGrafting Vitis vinifera on to Phylloxera resistant rootstocks is necessary in most wine growing regions of the world and therefore viticulture relies heavily upon successful graft union formation between the scion and the rootstock. The process of graft union formation begins with formation of a necrotic layer, followed by adhesion of the two grafted partners, callus cell proliferation and the establishment of a functional vascular system. Initially two-dimensional (2D) classical microscopy studies were performed to describe graft union formation. However, the establishment of graft union takes place in 3D and the lack of data in the third dimension makes it difficult to understand the process. In order to overcome this lack of information, 3D imaging techniques have been used, but with limited resolution. Here we will present methods to characterize the 3D morphological developments at the graft interface of grapevine using X-ray microtomography (micro-CT). This technique provides information on tissue architecture in relation with X-ray tissue absorbance and was used to visualize the 3D organization of callus and vascular tissues. In addition, we used iohexol, a contrast agent, to visualize functional xylem vessels connecting the scion and the rootstock. Images were compared before and 24 h after feeding iohexol to cut roots from where it moved to the shoot and labelled xylem vessels at the graft interface. These techniques were used to characterize xylem vessels connected between the scion and rootstock of grafts produced with different grafting techniques. © 2024 International Society for Horticultural Science. All rights reserved.
dc.language.isoENen_US
dc.subject.enGrapevine
dc.subject.enGrafting
dc.subject.enScion and rootstock interface
dc.subject.en3D imaging
dc.subject.enX-ray CT
dc.title.enImaging xylem connections between the scion and rootstock of grafted grapevine with X-ray microtomography
dc.typeCommunication dans un congrèsen_US
dc.identifier.doi10.17660/ACTAHORTIC.2024.1390.13en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.page103 – 108en_US
bordeaux.volume1390en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionINRAEen_US
bordeaux.conference.titleXI International Symposium on Grapevine Physiology and Biotechnologyen_US
bordeaux.countryzaen_US
bordeaux.conference.cityStellenboschen_US
hal.identifierhal-05025523
hal.version1
hal.date.transferred2025-04-08T13:54:14Z
hal.proceedingsouien_US
hal.conference.end2021-11-05
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2024-03&rft.volume=1390&rft.spage=103%20%E2%80%93%20108&rft.epage=103%20%E2%80%93%20108&rft.eissn=0567-7572&rft.issn=0567-7572&rft.au=JANOUEIX,%20Anne&SPILMONT,%20A.-S.&MOISY,%20C.&MATHIEU,%20G.&CORDELIERES,%20F.&rft.genre=unknown


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