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NMR resonance assignment of the cell death execution domain BELL2 from multicellular bacterial signalosomes

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDELCOURTE, Loic
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSANCHEZ, Corinne
dc.contributor.authorMORVAN, Estelle
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBERBON, Melanie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorGRELARD, Axelle
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSARAGAGLIA, Claire
dc.contributor.authorDAKHLI, Thierry
dc.contributor.authorTHORE, Stephane
dc.contributor.authorBARDIAUX, Benjamin
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorHABENSTEIN, Birgit
dc.contributor.authorKAUFFMANN, Brice
dc.contributor.authorSAUPE, Sven
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLOQUET, Antoine
dc.date.accessioned2025-02-14T10:36:55Z
dc.date.available2025-02-14T10:36:55Z
dc.date.issued2024-06-22
dc.identifier.issn1874-2718en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/204887
dc.description.abstractEnSignalosomes are high-order protein machineries involved in complex mechanisms controlling regulated immune defense and cell death execution. The immune response is initiated by the recognition of exogeneous or endogenous signals, triggering the signalosome assembly process. The final step of signalosome fate often involves membrane-targeting and activation of pore-forming execution domains, leading to membrane disruption and ultimately cell death. Such cell death-inducing domains have been thoroughly characterized in plants, mammals and fungi, notably for the fungal cell death execution protein domain HeLo. However, little is known on the mechanisms of signalosome-based immune response in bacteria, and the conformation of cell death executors in bacterial signalosomes is still poorly characterized. We recently uncovered the existence of NLR signalosomes in various multicellular bacteria and used genome mining approaches to identify putative cell death executors in Streptomyces olivochromogenes. These proteins contain a C-terminal amyloid domain involved in signal transmission and a N-terminal domain, termed BELL for Bacteria analogous to fungal HeLL (HeLo-like), presumably responsible for membrane-targeting, pore-forming and cell death execution. In the present study, we report the high yield expression of S. olivochromogenes BELL2 and its characterization by solution NMR spectroscopy. BELL is folded in solution and we report backbone and sidechain assignments. We identified five α-helical secondary structure elements and a folded core much smaller than its fungal homolog HeLo. This study constitutes the first step toward the NMR investigation of the full-length protein assembly and its membrane targeting.
dc.language.isoENen_US
dc.subject.enBELL
dc.subject.enCell death domain
dc.subject.enImmunity
dc.subject.enMulticellular bacteria
dc.subject.enSignalosome
dc.title.enNMR resonance assignment of the cell death execution domain BELL2 from multicellular bacterial signalosomes
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s12104-024-10183-5en_US
dc.subject.halChimie/Matériauxen_US
dc.identifier.pubmed38907837en_US
bordeaux.journalBiomolecular Nmr Assignmentsen_US
bordeaux.page159-164en_US
bordeaux.volume18en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.issue2en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomolecular%20Nmr%20Assignments&rft.date=2024-06-22&rft.volume=18&rft.issue=2&rft.spage=159-164&rft.epage=159-164&rft.eissn=1874-2718&rft.issn=1874-2718&rft.au=DELCOURTE,%20Loic&SANCHEZ,%20Corinne&MORVAN,%20Estelle&BERBON,%20Melanie&GRELARD,%20Axelle&rft.genre=article


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