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Inhibiting Autophagy by Chemicals During SCAPs Osteodifferentiation Elicits Disorganized Mineralization, While the Knock-Out of Atg5/7 Genes Leads to Cell Adaptation

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLE NIHOUANNEN, Damien
ORCID: 0000-0003-1539-5183
IDREF: 102521751
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOIZIAU, Claudine
ORCID: 0000-0002-0475-2571
IDREF: 85228370
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorREY, Sylvie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorAGADZHANIAN, Nicole
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDUSSERRE, Nathalie
dc.contributor.authorCORDELIÈRES, Fabrice
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorPRIAULT, Muriel
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOEUF, Helene
dc.date.accessioned2025-01-23T15:23:04Z
dc.date.available2025-01-23T15:23:04Z
dc.date.issued2025-01-20
dc.identifier.issn2073-4409en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/204527
dc.description.abstractEnSCAPs (Stem Cells from Apical Papilla), derived from the apex of forming wisdom teeth, extracted from teenagers for orthodontic reasons, belong to the MSCs (Mesenchymal Stromal Cells) family. They have multipotent differentiation capabilities and are a potentially powerful model for investigating strategies of clinical cell therapies. Since autophagy—a regulated self-eating process—was proposed to be essential in osteogenesis, we investigated its involvement in the SCAP model. By using a combination of chemical and genetic approaches to inhibit autophagy, we studied early and late events of osteoblastic differentiation. We showed that blocking the formation of autophagosomes with verteporfin did not induce a dramatic alteration in early osteoblastic differentiation monitored by ALP (alkaline phosphatase) activity. However, blocking the autophagy flux with bafilomycin A1 led to ALP repression. Strikingly, the mineralization process was observed with both compounds, with calcium phosphate (CaP) nodules that remained inside cells under bafilomycin A1 treatment and numerous but smaller CaP nodules after verteporfin treatment. In contrast, deletion of Atg5 or Atg7, two genes involved in the formation of autophagosomes and essential to trigger canonical autophagy, indicated that both genes could be involved differently in the mineralization process with a modification of the ALP activity while final mineralization was not altered.
dc.description.sponsorshipDéveloppment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004en_US
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enMesenchymal Stem Cells
dc.subject.enScaps
dc.subject.enCanonical Autophagy
dc.subject.enAlternative Autophagy
dc.subject.enBafilomycin A1
dc.subject.enVerteporfin
dc.subject.enLC3
dc.subject.enALP Activity
dc.subject.enOsteoblasts
dc.subject.enMineralization
dc.title.enInhibiting Autophagy by Chemicals During SCAPs Osteodifferentiation Elicits Disorganized Mineralization, While the Knock-Out of Atg5/7 Genes Leads to Cell Adaptation
dc.title.alternativeCellsen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/cells14020146en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaireen_US
bordeaux.journalCellsen_US
bordeaux.page146en_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026en_US
bordeaux.issue2en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDFondation des Gueules Casséesen_US
bordeaux.import.sourcecrossref
hal.identifierhal-04908968
hal.version1
hal.date.transferred2025-01-23T15:23:08Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcecrossref
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cells&rft.date=2025-01-20&rft.volume=14&rft.issue=2&rft.spage=146&rft.epage=146&rft.eissn=2073-4409&rft.issn=2073-4409&rft.au=LE%20NIHOUANNEN,%20Damien&BOIZIAU,%20Claudine&REY,%20Sylvie&AGADZHANIAN,%20Nicole&DUSSERRE,%20Nathalie&rft.genre=article


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