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Encapsidation of RNA-Polyelectrolyte Complexes with Amphiphilic Block Copolymers: Toward a New Self-Assembly Route

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorBUI, Laurent
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorABBOU, Scarlette
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorIBARBOURE, Emmanuel
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorGUIDOLIN, Nicolas
hal.structure.identifierARN : régulations naturelle et artificielle
hal.structure.identifierImagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB]
dc.contributor.authorSTAEDEL, Cathy
hal.structure.identifierARN : régulations naturelle et artificielle
hal.structure.identifierImagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB]
dc.contributor.authorTOULMÉ, Jean-Jacques
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorSCHATZ, Christophe
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2012
dc.identifier.issn0002-7863
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20450
dc.description.abstractEnAmphiphilic block copolymers are molecules composed of hydrophilic and hydrophobic segments having the capacity to spontaneously self-assemble into a variety of supramolecular structures like micelles and vesicles. Here, we propose an original way to self-assemble amphiphilic block copolymers into a supported bilayer membrane for defined coating of nanoparticles. The heart of the method rests on a change of the amphiphilicity of the copolymer that can be turned off and on by varying the polarity of the solvent. In this condition, the assembly process can take advantage of specific molecular interactions in both organic solvent and water. While the concept potentially could be applied to any type of charged substrates, we focus our interest on the design of a new type of polymer assembly mimicking the virus morphology. A capsid-like shell of glycoprotein-mimic amphiphilic block copolymer was self-assembled around a positively charged complex of siRNA and polyethyleneimine. The process requires two steps. Block copolymers first interact with the complexes dispersed in DMSO through electrostatic interactions. Next, the increase of the water content in the medium triggers the hydrophobic effect and the concomitant self-assembly of free block copolymer molecules into a bilayer membrane at the complex surface. The higher gene silencing activity of the copolymer-modified complexes over the complexes alone shows the potential of this new type of nanoconstructs for biological applications, especially for the delivery of therapeutic biomolecules.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subject.enPLASMID DNA
dc.subject.enDRUG-DELIVERY
dc.subject.enSIRNA DELIVERY
dc.subject.enGENE DELIVERY
dc.subject.enNUCLEIC-ACID DELIVERY
dc.subject.enVIRUS
dc.subject.enPOLYPLEXES
dc.subject.enPOLYMERSOMES
dc.title.enEncapsidation of RNA-Polyelectrolyte Complexes with Amphiphilic Block Copolymers: Toward a New Self-Assembly Route
dc.typeArticle de revue
dc.identifier.doi10.1021/ja310397j
dc.subject.halChimie/Polymères
bordeaux.journalJournal of the American Chemical Society
bordeaux.page20189-20196
bordeaux.volume134
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue49
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-00805009
hal.version1
dc.subject.esVESICLES
dc.subject.esLIPOPLEXES
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00805009v1
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