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Glucose-induced remodeling of intermediary and energy metabolism in procyclic Trypanosoma brucei.

dc.rights.licenseopenen_US
dc.contributor.authorCOUSTOU, Virginie
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorBIRAN, Marc
hal.structure.identifierUnité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
hal.structure.identifierInstitut de Biologie du Développement de Marseille [IBDM]
dc.contributor.authorBRETON, Marc
dc.contributor.authorGUEGAN, Fabien
dc.contributor.authorRIVIÈRE, Loïc
dc.contributor.authorPLAZOLLES, Nicolas
dc.contributor.authorNOLAN, Derek
dc.contributor.authorBARRETT, Michael P
hal.structure.identifierCentre de résonance magnétique des systèmes biologiques [CRMSB]
dc.contributor.authorFRANCONI, Jean-Michel
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBRINGAUD, Frédéric
dc.date.accessioned2025-01-14T08:26:04Z
dc.date.available2025-01-14T08:26:04Z
dc.date.issued2008-06-13
dc.identifier.issn0021-9258en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/204250
dc.description.abstractEnThe procyclic form of Trypanosoma brucei is a parasitic protozoan that normally dwells in the midgut of its insect vector. In vitro, this parasite prefers d-glucose to l -proline as a carbon source, although this amino acid is the main carbon source available in its natural habitat. Here, we investigated how l -proline is metabolized in glucose-rich and glucose-depleted conditions. Analysis of the excreted end products of (13)C-enriched l -proline metabolism showed that the amino acid is converted into succinate or l -alanine depending on the presence or absence of d-glucose, respectively. The fact that the pathway of l -proline metabolism was truncated in glucose-rich conditions was confirmed by the analysis of 13 separate RNA interference-harboring or knock-out cell lines affecting different steps of this pathway. For instance, RNA interference studies revealed the loss of succinate dehydrogenase activity to be conditionally lethal only in the absence of d-glucose, confirming that in glucose-depleted conditions, l -proline needs to be converted beyond succinate. In addition, depletion of the F(0)/F(1)-ATP synthase activity by RNA interference led to cell death in glucose-depleted medium, but not in glucose-rich medium. This implies that, in the presence of d-glucose, the importance of the F(0)/F(1)-ATP synthase is diminished and ATP is produced by substrate level phosphorylation. We conclude that trypanosomes develop an elaborate adaptation of their energy production pathways in response to carbon source availability.
dc.language.isoENen_US
dc.subject.meshAcetyl Coenzyme A
dc.subject.meshAdenosine Triphosphate
dc.subject.meshPhosphorylation
dc.subject.meshProline
dc.subject.meshProton-Translocating ATPases
dc.subject.meshRNA Interference
dc.subject.meshTrypanosoma brucei brucei
dc.subject.meshAnimals
dc.subject.meshCarbon
dc.subject.meshCulture Media
dc.subject.meshGene Expression Regulation
dc.subject.meshGlucose
dc.subject.meshMagnetic Resonance Spectroscopy
dc.subject.meshMitochondria
dc.subject.meshModels, Biological
dc.title.enGlucose-induced remodeling of intermediary and energy metabolism in procyclic Trypanosoma brucei.
dc.typeArticle de revueen_US
dc.identifier.doi10.1074/jbc.M709592200en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed18430732en_US
bordeaux.journalJournal of Biological Chemistryen_US
bordeaux.page16342-54en_US
bordeaux.volume283en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue24en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-00318595
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Biological%20Chemistry&rft.date=2008-06-13&rft.volume=283&rft.issue=24&rft.spage=16342-54&rft.epage=16342-54&rft.eissn=0021-9258&rft.issn=0021-9258&rft.au=COUSTOU,%20Virginie&BIRAN,%20Marc&BRETON,%20Marc&GUEGAN,%20Fabien&RIVI%C3%88RE,%20Lo%C3%AFc&rft.genre=article


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