Simultaneous enzyme grafting on bio-inspired scaffolds for antibacterial protection
| dc.rights.license | open | en_US |
| dc.contributor.author | ARBEZ, Baptiste | |
| dc.contributor.author | RETOURNEY, Chloe | |
| dc.contributor.author | QUILES, Fabienne | |
| dc.contributor.author | FRANCIUS, Gregory | |
| dc.contributor.author | FIEROBE, Henri-Pierre | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | EL-KIRAT-CHATEL, Sofiane | |
| dc.date.accessioned | 2025-01-13T15:07:15Z | |
| dc.date.available | 2025-01-13T15:07:15Z | |
| dc.date.issued | 2024 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/204245 | |
| dc.description.abstractEn | Surface bacterial contamination represents a crucial health and industrial concern which requires new strategies to be continuously developed. Successful antibacterial surfaces are characterized by a combination of durable and broad-spectrum antimicrobial actions. Herein, we present a bio-inspired strategy mimicking natural cellulosome to simultaneously immobilize multiple enzymes with antibacterial activity onto surfaces. The grafting strategy leverages the strong biomolecular interaction between receptors on a scaffold protein anchored on the substrate and ligands added to the enzymes. As a proof of concept, lysozyme and lysostaphin were chosen to target the bacterial cell wall, and DNase I to degrade DNA released during cell lysis, known to promote bacterial adhesion which can later lead to biofilm formation. The specificity of the ligand/receptor interaction was confirmed by biochemical and AFM-based single-molecule force spectroscopy assays, thus demonstrating successful co-immobilization of the three enzymes on the protein scaffold. Then, the antibacterial protection was evaluated against Staphylococcus aureus, Escherichia coli and Micrococcus luteus by viability tests which revealed long-term antimicrobial protection of the multi-enzymatic scaffold on both Gram-positive and Gram-negative bacteria. After 24 hours of contact, the system induced lysis of 71 to 85% of bacteria, and its antimicrobial properties remained effective after 5 days even with several cumulative waves of bacterial contamination. This work demonstrates the relevance of bio-inspired multi-enzymatic scaffolds for antibacterial protection, providing long-term and broad-spectrum action. | |
| dc.description.sponsorship | Armatures multienzymatiques pour surfaces antimicrobiennes bioinspirées - ANR-20-CE06-0001 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.title.en | Simultaneous enzyme grafting on bio-inspired scaffolds for antibacterial protection | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1039/d3ma00703k | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | Materials Advances | en_US |
| bordeaux.page | 1171 | en_US |
| bordeaux.volume | 5 | en_US |
| bordeaux.hal.laboratories | CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| dc.rights.cc | CC BY-NC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Materials%20Advances&rft.date=2024&rft.volume=5&rft.spage=1171&rft.epage=1171&rft.au=ARBEZ,%20Baptiste&RETOURNEY,%20Chloe&QUILES,%20Fabienne&FRANCIUS,%20Gregory&FIEROBE,%20Henri-Pierre&rft.genre=article |
