Mostrar el registro sencillo del ítem

Mobilization of an ICEclc-like element as a potential mechanism for the spread of IMP-13 carbapenemase in Pseudomonas aeruginosa.

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBIENTZ, Léa
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
hal.structure.identifierCentre de Bioinformatique de Bordeaux [CBIB]
dc.contributor.authorGUYET, Ulysse
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorGUIRAUD, Jennifer
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMETIFIOT, Mathieu
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMOULIERAS, Mikeldi
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorAILLERIE, Sabine
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorCOULANGE-MAYONNOVE, Laure
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorABDOU, Bachir Boureima
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
hal.structure.identifierCentre de Bioinformatique de Bordeaux [CBIB]
dc.contributor.authorGROPPI, Alexis
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
hal.structure.identifierCentre de Bioinformatique de Bordeaux [CBIB]
dc.contributor.authorNIKOLSKI, Macha
IDREF: 134506049
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBÉBÉAR, Cécile
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPEREYRE, Sabine
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorDUBOIS, Véronique
dc.date.accessioned2025-01-07T13:48:15Z
dc.date.available2025-01-07T13:48:15Z
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/204167
dc.description.abstractEnCarbapenem-resistant Pseudomonas aeruginosa is a global public health concern. IMP-13 is a carbapenemase that was described for the first time in 2001 but is often underestimated due to poor hydrolysis of carbapenems and a lack of molecular detection. The aim of this study was to characterize the genetic support of bla in P. aeruginosa and to assess the ability of mobile genetic elements to disseminate this resistance. A retrospective analysis conducted between 2010 and 2020 revealed eight multiresistant P. aeruginosa isolates by their production of the carbapenemase IMP-13 in Bordeaux. Additionally, three of the studied isolates exhibited high-level resistance to imipenem and imipenem-relebactam that was linked to an insertion sequence in the oprD gene. Successful mating was achieved, and transconjugants and parental clinical isolate genomes were sequenced. All clinical isolates were found to be ST621 strains. The data revealed that bla was carried on an Integrative and Conjugative Element (ICEclc-like) of 88589 bp with a 62% GC content harboring 85 CDSs, and was inserted at the tRNA locus PA0729.1. The ICE was identical in the eight studied clinical isolates and in all the ST621 strains found in the databases. The conjugation rate was low, at approximately 10 transconjugants per donor and ICE transfer appeared to mobilize some adjacent parental genes located immediately downstream of the ICE. In conclusion, these results suggest that even if the spread of bla is mainly due to an epidemic ST621 clone, the mobilization of a bla-carrying ICEclc-like element is possible and should not be underestimated.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectICEclc-like element; IMP-13 carbapenemase; Pseudomonas aeruginosa; antibiotic resistance; horizontal gene transfer.
dc.title.enMobilization of an ICEclc-like element as a potential mechanism for the spread of IMP-13 carbapenemase in Pseudomonas aeruginosa.
dc.typeDocument de travail - Pré-publicationen_US
dc.identifier.doi10.1016/j.jgar.2024.12.006en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed39706477en_US
bordeaux.journalJournal of Global Antimicrobial Resistanceen_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRSen_US
bordeaux.import.sourcepubmed
hal.identifierhal-04870893
hal.version1
hal.date.transferred2025-01-07T13:48:18Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.subtypePrepublication/Preprinten_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Global%20Antimicrobial%20Resistance&rft.au=BIENTZ,%20L%C3%A9a&GUYET,%20Ulysse&GUIRAUD,%20Jennifer&METIFIOT,%20Mathieu&MOULIERAS,%20Mikeldi&rft.genre=preprint


Archivos en el ítem

Thumbnail
Nombre:
MFP_J.Glob.Antimicrob.Resist ...
Tamaño:
1.319Mb
Formato:
PDF
Ver/Abrir
Thumbnail
Nombre:
license_rdf
Tamaño:
914bytes
Formato:
application/rdf+xml
Ver/Abrir

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem