Determination of glycation biomarkers in human fingernails by isotope-dilution liquid chromatography tandem mass spectrometry (LC-MS/MS)
| dc.rights.license | open | en_US |
| dc.contributor.author | TESSIER, Frederic J | |
| dc.contributor.author | ORTIZ, Sarahi Jaramillo | |
| dc.contributor.author | NGUYEN, Dinh Hieu | |
| dc.contributor.author | MOHAMMEDI, Kamel | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| dc.contributor.author | DELCOURT, Cecile | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| dc.contributor.author | HELMER, Catherine | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| dc.contributor.author | GOFF, Melanie Le | |
| dc.contributor.author | BOULANGER, Eric | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| dc.contributor.author | RIGALLEAU, Vincent
IDREF: 069788146 | |
| dc.contributor.author | HOWSAM, Michael | |
| dc.date.accessioned | 2024-12-18T15:04:20Z | |
| dc.date.available | 2024-12-18T15:04:20Z | |
| dc.date.issued | 2025-01-30 | |
| dc.identifier.issn | 1873-3492 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/203988 | |
| dc.description.abstractEn | Glycation is a non-enzymatic, post-translational modification of proteins which is elevated in several pathologies, notably diabetes. An early-stage glycation product, glycated hemoglobin (HbA1c), is used in the clinical management of diabetes, and advanced glycation end-products (AGEs) are implicated in the etiology of diabetic complications. Fingernail clippings contain a time-integrated repository of several metabolic processes during the preceding 3-5 months, are easily sampled, and various elements and molecules have been shown to remain stable within them for long periods without refrigeration. Building upon a few underexploited studies, we investigated fingernails as a non-invasive matrix to assess glycation using liquid chromatography-mass spectrometry to quantify ungual biomarkers of early- and advanced glycation (respectively furosine, as a fructose-lysine derivative, and two AGEs (N(ε)-carboxymethyllysine (CML) and N(ε)-carboxyethyllysine (CEL)). The method was appropriately validated and provided accurate and precise measurements of two amino acids and the glycation biomarkers. Sample storage at ± 25 °C for 12 months had no effect upon these analytes, and the method was applied to fingernails from 87 people with diabetes. There was a moderate, linear correlation between ungual furosine concentrations and HbA1c at the time of nail sampling (r(s) = 0.339, p = 0.0011). Among subjects for whom previous measurements were available, there was no correlation between ungual glycation and HbA1c measured > 3 months before nail sampling, indicating that ungual furosine reflects early-stage glycation over a similar period to HbA1c. This study provides further evidence, using modern analytical techniques, that fingernails offer the possibility to quantitatively and non-invasively assess glycation. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Biomarkers | |
| dc.subject.en | Diabetes | |
| dc.subject.en | Fingernails | |
| dc.subject.en | Glycation | |
| dc.subject.en | HbA1c | |
| dc.subject.en | Mass spectrometry | |
| dc.title.en | Determination of glycation biomarkers in human fingernails by isotope-dilution liquid chromatography tandem mass spectrometry (LC-MS/MS) | |
| dc.title.alternative | Clin Chim Acta | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.cca.2024.120036 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Santé publique et épidémiologie | en_US |
| dc.identifier.pubmed | 39551231 | en_US |
| bordeaux.journal | Clinica Chimica Acta | en_US |
| bordeaux.page | 120036 | en_US |
| bordeaux.volume | 566 | en_US |
| bordeaux.hal.laboratories | Bordeaux Population Health Research Center (BPH) - UMR 1219 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.team | LEHA_BPH | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-04846367 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-12-18T15:04:22Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Clinica%20Chimica%20Acta&rft.date=2025-01-30&rft.volume=566&rft.spage=120036&rft.epage=120036&rft.eissn=1873-3492&rft.issn=1873-3492&rft.au=TESSIER,%20Frederic%20J&ORTIZ,%20Sarahi%20Jaramillo&NGUYEN,%20Dinh%20Hieu&MOHAMMEDI,%20Kamel&DELCOURT,%20Cecile&rft.genre=article |
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