Elucidating the Transport Mechanisms and Metabolic Roles of Serine, Threonine, and Glycine in Trypanosoma cruzi
| dc.rights.license | open | en_US |
| hal.structure.identifier | Universidade de São Paulo = University of São Paulo [USP] | |
| dc.contributor.author | ALENCAR, Mayke Bezerra | |
| hal.structure.identifier | Universidade de São Paulo = University of São Paulo [USP] | |
| dc.contributor.author | BRUNO MOREIRA GIRARD, Richard Marcel | |
| hal.structure.identifier | Universidade de São Paulo = University of São Paulo [USP] | |
| dc.contributor.author | CRISPIM, Marcell | |
| hal.structure.identifier | Universidade de São Paulo = University of São Paulo [USP] | |
| dc.contributor.author | BAPTISTA, Carlos Gustavo | |
| hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
| dc.contributor.author | BIRAN, Marc | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BRINGAUD, Frédéric | |
| hal.structure.identifier | Universidade de São Paulo = University of São Paulo [USP] | |
| dc.contributor.author | SILBER, Ariel Mariano | |
| dc.date.accessioned | 2024-11-06T08:00:10Z | |
| dc.date.available | 2024-11-06T08:00:10Z | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/203138 | |
| dc.description.abstractEn | L-Serine (L-Ser) and L-Threonine (L-Thr) have versatile roles in metabolism. In addition to their use in protein synthesis, these amino acids participate in the biosynthesis pathways of other amino acids and even phospholipids. Furthermore, L-Ser and L-Thr can be substrates for a Ser/Thr dehydratase (Ser/ThrDH), resulting in pyruvate (Pyr) and 2-oxobutyrate, respectively, thus being amino acids with anaplerotic potential. Trypanosoma cruzi, the etiological agent of Chagas disease, uses amino acids in several biological processes: metacyclogenesis, infection, resistance to nutritional and oxidative stress, osmotic control, etc. In this study, we investigated the import and metabolism of L-Ser, L-Thr, and Gly in T. cruzi. Our results demonstrate that these amino acids are transported from the extracellular environment into T. cruzi cells through a saturable transport system that fits the Michaelis-Menten model. Our results show that L-Ser and L-Thr can sustain epimastigote (Epi) cell viability under nutritional stress (NS) conditions and can stimulate oxygen consumption to maintain intracellular ATP levels. Additionally, our findings indicate that l-Ser plays a role in establishing the mitochondrial membrane potential (ΔΨm) in T. cruzi. L-Ser is also involved in energy metabolism via the Ser-Pyr pathway, which stimulates the production and subsequent excretion of acetate and alanine. Our results demonstrate the importance of L-Ser and l-Thr in the energy metabolism of T. cruzi and provide new insights into the metabolic adaptations of this parasite during its life cycle | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Trypanosoma cruzi | |
| dc.subject.en | amino acid metabolism | |
| dc.subject.en | transport | |
| dc.subject.en | bioenergetics | |
| dc.subject.en | nutritional stress | |
| dc.title.en | Elucidating the Transport Mechanisms and Metabolic Roles of Serine, Threonine, and Glycine in Trypanosoma cruzi | |
| dc.type | Document de travail - Pré-publication | en_US |
| dc.identifier.doi | 10.1101/2024.06.29.601350 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Parasitologie | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC] | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.import.source | hal | |
| hal.identifier | hal-04765838 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.subtype | Prepublication/Preprint | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=ALENCAR,%20Mayke%20Bezerra&BRUNO%20MOREIRA%20GIRARD,%20Richard%20Marcel&CRISPIM,%20Marcell&BAPTISTA,%20Carlos%20Gustavo&BIRAN,%20Marc&rft.genre=preprint |
