Detection and Characterization of Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae from Hospital Effluents of Ouagadougou, Burkina Faso
| dc.rights.license | open | en_US |
| dc.contributor.author | KAGAMBÈGA, Alix Bénédicte | |
| dc.contributor.author | DEMBÉLÉ, René | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BIENTZ, Léa | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | M’ZALI, Fatima | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MAYONNOVE, Laure | |
| dc.contributor.author | MOHAMED, Alassane Halawen | |
| dc.contributor.author | COULIBALY, Hiliassa | |
| dc.contributor.author | BARRO, Nicolas | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | DUBOIS, Véronique | |
| dc.date.accessioned | 2024-10-30T14:53:22Z | |
| dc.date.available | 2024-10-30T14:53:22Z | |
| dc.date.issued | 2023-09-29 | |
| dc.identifier.issn | 2079-6382 | en_US |
| dc.identifier.uri | oai:crossref.org:10.3390/antibiotics12101494 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/203007 | |
| dc.description.abstractEn | Hospital wastewater is a recognized reservoir for resistant Gram-negative bacteria. This study aimed to screen for carbapenemase-producing Escherichia coli and Klebsiella pneumoniae and their resistance determinants in two hospital effluents of Ouagadougou. Carbapenem-resistant E. coli and K. pneumoniae were selectively isolated from wastewater collected from two public hospitals in Ouagadougou, Burkina Faso. Bacterial species were identified via MALDI-TOF mass spectrometry. Carbapenemase production was studied phenotypically using antibiotic susceptibility testing via the disk diffusion method. The presence of carbapenemases was further characterized by PCR. A total of 14 E. coli (13.59%) and 19 K. pneumoniae (17.92%) carbapenemase-producing isolates were identified with different distributions. They were, respectively, blaNDM (71.43%), blaVIM (42.86%), blaIMP (28.57%), blaKPC (14.29%), blaOXA-48 (14.29%); and blaKPC (68.42%), blaNDM (68.42%), blaIMP (10.53%), blaVIM (10.53%), and blaOXA-48 (5.26%). In addition, eight (57.14%) E. coli and eleven (57.89%) K. pneumoniae isolates exhibited more than one carbapenemase, KPC and NDM being the most prevalent combination. Our results highlight the presence of clinically relevant carbapenemase-producing isolates in hospital effluents, suggesting their presence also in hospitals. Their spread into the environment via hospital effluents calls for intensive antimicrobial resistance (AMR) surveillance. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.source | crossref | |
| dc.subject.en | Escherichia coli; Klebsiella pneumoniae; Ouagadougou; carbapenemases; hospital effluents. | |
| dc.title.en | Detection and Characterization of Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae from Hospital Effluents of Ouagadougou, Burkina Faso | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/antibiotics12101494 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 37887195 | en_US |
| bordeaux.journal | Antibiotics (Basel, Switzerland) | en_US |
| bordeaux.page | 1494 | en_US |
| bordeaux.volume | 12 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 10 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | dissemin | |
| hal.identifier | hal-04760621 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-10-30T14:53:25Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | dissemin | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Antibiotics%20(Basel,%20Switzerland)&rft.date=2023-09-29&rft.volume=12&rft.issue=10&rft.spage=1494&rft.epage=1494&rft.eissn=2079-6382&rft.issn=2079-6382&rft.au=KAGAMB%C3%88GA,%20Alix%20B%C3%A9n%C3%A9dicte&DEMB%C3%89L%C3%89,%20Ren%C3%A9&BIENTZ,%20L%C3%A9a&M%E2%80%99ZALI,%20Fatima&MAYONNOVE,%20Laure&rft.genre=article |
