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Development of a rapid antibody test for point-of-care diagnosis of animal African trypanosomosis

dc.rights.licenseopenen_US
dc.contributor.authorBOULANGÉ, Alain
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPILLAY, Davita
dc.contributor.authorCHEVTZOFF, Cyrille
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBITEAU, Nicolas
dc.contributor.authorCOMÉ DE GRAÇA, Vanessa
dc.contributor.authorREMPETERS, Leonie
dc.contributor.authorTHEODORIDIS, Dimitrios
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBALTZ, Théo
dc.date.accessioned2024-10-30T14:46:06Z
dc.date.available2024-10-30T14:46:06Z
dc.date.issued2017-01-01
dc.identifier.issn0304-4017en_US
dc.identifier.urihttp://agritrop.cirad.fr/583090/
dc.identifier.urioai:crossref.org:10.1016/j.vetpar.2016.11.017
dc.identifier.uriftcirad:oai:agritrop.cirad.fr:583090
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/203005
dc.description.abstractEnTrypanosoma congolense and T. vivax are the main causative agents of animal African trypanosomosis (AAT), a disease which hinders livestock production throughout sub-Saharan Africa and in some parts of South America. Although two trypanocidal drugs are currently available, the level of treatment is low due to the difficulty in diagnosing the disease in the field. The major clinical signs of AAT such as anaemia, weight loss, and infertility, are common to several other endemic livestock diseases. Current diagnostic methods, based on the visualization of the parasite in the blood, or on the detection of its DNA or the antibodies it triggers in the host, are not suitable for direct use in the field as they require specialized equipment and personnel. Thus, we developed a quick-format diagnostic test (15 min) based on the recombinant TcoCB and TvGM6 antigens for detection of T. congolense and T. vivax, respectively, aimed at providing farmers and veterinarians in the field with the means to conduct a quick diagnosis. The specificity and sensitivity of the test were evaluated using sera from experimentally infected cattle, and fresh blood when possible. The prototype, which includes both antigens, shows a specificity of 95.9 (95% C.I., 90.4%–100%) and a sensitivity of 92.0% (95% C.I., 85.9%–98.1%) for T. congolense and 98.2% (95% C.I., 94.7%–100%) for T. vivax. The high levels of sensitivity and specificity of this rapid test, the possibility of using directly whole blood, and the ease of interpreting the result, all contribute to make of this test a valuable candidate to contribute to the control of AAT in the field. However, further tests with more representative, numerous and fresh reference samples are necessary in order to compare this test with the ELISA, the current gold standard serological test for trypanosomosis. (Résumé d'auteur)
dc.language.isoENen_US
dc.sourcecrossref
dc.sourcebase
dc.subject.enAfrican animal trypanosomosis; Bovine; Rapid Diagnostic Test (RDT); Trypanosoma congolense; Trypanosoma vivax.
dc.title.enDevelopment of a rapid antibody test for point-of-care diagnosis of animal African trypanosomosis
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.vetpar.2016.11.017en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed28043385en_US
bordeaux.journalVeterinary Parasitologyen_US
bordeaux.page32-38en_US
bordeaux.volume233en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04760600
hal.version1
hal.date.transferred2024-10-30T14:46:08Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Veterinary%20Parasitology&rft.date=2017-01-01&rft.volume=233&rft.spage=32-38&rft.epage=32-38&rft.eissn=0304-4017&rft.issn=0304-4017&rft.au=BOULANG%C3%89,%20Alain&PILLAY,%20Davita&CHEVTZOFF,%20Cyrille&BITEAU,%20Nicolas&COM%C3%89%20DE%20GRA%C3%87A,%20Vanessa&rft.genre=article


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