Mostrar el registro sencillo del ítem

The cell-assembled extracellular matrix: A focus on the storage stability and terminal sterilization of this human “bio” material

dc.rights.licenseopenen_US
dc.contributor.authorPOTART, D.
dc.contributor.authorGLUAIS, M.
dc.contributor.authorGAUBERT, A.
dc.contributor.authorDA SILVA, N.
dc.contributor.authorHOURQUES, M.
dc.contributor.authorSARRAZIN, M.
dc.contributor.authorIZOTTE, J.
dc.contributor.authorMORA CHARROT, L.
dc.contributor.authorL'HEUREUX, N.
dc.date.accessioned2024-10-24T08:14:44Z
dc.date.available2024-10-24T08:14:44Z
dc.date.issued2023
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/202697
dc.description.abstractEnThe Cell-Assembled extracellular Matrix (CAM) is an attractive biomaterial because it provided the backbone of vascular grafts that were successfully implanted in patients, and because it can now be assembled in “human textiles”. For future clinical development, it is important to consider key manufacturing questions. In this study, the impact of various storage conditions and sterilization methods were evaluated. After 1 year of dry frozen storage, no change in mechanical nor physicochemical properties were detected. However, storage at 4 °C and room temperature resulted in some mechanical changes, especially for dry CAM, but physicochemical changes were minor. Sterilization modified CAM mechanical and physicochemical properties marginally except for hydrated gamma treatment. All sterilized CAM supported cell proliferation. CAM ribbons were implanted subcutaneously in immunodeficient rats to assess the impact of sterilization on the innate immune response. Sterilization accelerated strength loss but no significant difference could be shown at 10 months. Very mild and transient inflammatory responses were observed. Supercritical CO2 sterilization had the least effect. In conclusion, the CAM is a promising biomaterial since it is unaffected by long-term storage in conditions available in hospitals (hydrated at 4 °C), and can be sterilized terminally (scCO2) without compromising in vitro nor in vivo performance. Statement of significance: In the field of tissue engineering, the use of extracellular matrix (ECM) proteins as a scaffolding biomaterial has become very popular. Recently, many investigators have focused on ECM produced by cells in vitro to produce unprocessed biological scaffolds. As this new kind of “biomaterial” becomes more and more relevant, it is critical to consider key manufacturing questions to facilitate future transition to the clinic. This article presents an extensive evaluation of long-term storage stability and terminal sterilization effects on an extracellular matrix assembled by cells in vitro. We believe that this article will be of great interest to help tissue engineers involved in so-called scaffold-free approaches to better prepare the translation from benchtop to bedside. © 2023
dc.language.isoENen_US
dc.title.enThe cell-assembled extracellular matrix: A focus on the storage stability and terminal sterilization of this human “bio” material
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.actbio.2023.05.002en_US
dc.subject.halSciences du Vivant [q-bio]en_US
bordeaux.journalActa Biomaterialiaen_US
bordeaux.page133-146en_US
bordeaux.volume166en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.description.error<?xml version="1.0" encoding="utf-8"?> <sword:error xmlns:sword="http://purl.org/net/sword/error/" xmlns="http://www.w3.org/2005/Atom" href="http://purl.org/net/sword/error/ErrorBadRequest"> <title>ERROR</title> <updated>2024-10-25T01:04:48+02:00</updated> <author> <name>HAL SWORD API Server</name> </author> <source> <generator uri="https://api.archives-ouvertes.fr/sword" version="1.0">hal@ccsd.cnrs.fr</generator> </source> <summary>Some parameters sent with the request were not understood</summary> <sword:treatment>processing failed</sword:treatment> <sword:verboseDescription>{"author":["Au moins 1 auteur doit \u00eatre affili\u00e9"]}</sword:verboseDescription> <link rel="alternate" href="https://api.archives-ouvertes.fr" type="text/html"/> </sword:error>
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Acta%20Biomaterialia&amp;rft.date=2023&amp;rft.volume=166&amp;rft.spage=133-146&amp;rft.epage=133-146&amp;rft.au=POTART,%20D.&amp;GLUAIS,%20M.&amp;GAUBERT,%20A.&amp;DA%20SILVA,%20N.&amp;HOURQUES,%20M.&amp;rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem