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Rubber particle proteins, HbREF and HbSRPP, show different interactions with model membranes

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorBERTHELOT, Karine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLECOMTE, Sophie
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorESTEVEZ, Yannick
hal.structure.identifierSoutien à la Recherche de l'Institut Européen de Chimie Biologique
dc.contributor.authorZHENDRE, Vanessa
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorHENRY, Sarah
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorTHÉVENOT, Julie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierSoutien à la Recherche de l'Institut Européen de Chimie Biologique
dc.contributor.authorDUFOURC, Eric J.
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorALVES, Isabel
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorPERUCH, Frédéric
IDREF: 152900748
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2014
dc.identifier.issn0005-2736
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20230
dc.description.abstractEnThe biomembrane surrounding rubber particles from the hevea latex is well known for its content of numerous allergen proteins. HbREF (Hevb1) and HbSRPP (Hevb3) are major components, linked on rubber particles, and they have been shown to be involved in rubber synthesis or quality (mass regulation), but their exact function is still to be determined. In this study we highlighted the different modes of interactions of both recombinant proteins with various membrane models (lipid monolayers, liposomes or supported bilayers, and multilamellar vesicles) to mimic the latex particle membrane. We combined various biophysical methods (polarization-modulation-infrared reflection-adsorption spectroscopy (PM-IRRAS)/ellipsometry, attenuated-total reflectance Fourier-transform infrared (ATR-FTIR), solid-state nuclear magnetic resonance (NMR), plasmon waveguide resonance (PWR), fluorescence spectroscopy) to elucidate their interactions. Small rubber particle protein (SRPP) shows less affinity than rubber elongation factor (REF) for the membranes but displays a kind of "covering" effect on the lipid headgroups without disturbing the membrane integrity. Its structure is conserved in the presence of lipids. Contrarily, REF demonstrates higher membrane affinity with changes in its aggregation properties, the amyloid nature of REF, which we previously reported, is not favored in the presence of lipids. REF binds and inserts into membranes. The membrane integrity is highly perturbed, and we suspect that REF is even able to remove lipids from the membrane leading to the formation of mixed micelles. These two homologous proteins show affinity to all membrane models tested but neatly differ in their interacting features. This could imply differential roles on the surface of rubber particles.
dc.language.isoen
dc.publisherElsevier
dc.subject.enSRPP
dc.subject.enHevea brasiliensis latex
dc.subject.enNatural rubber
dc.subject.enPlant membrane
dc.subject.enREF
dc.title.enRubber particle proteins, HbREF and HbSRPP, show different interactions with model membranes
dc.typeArticle de revue
dc.identifier.doi10.1016/j.bbamem.2013.08.025
dc.subject.halChimie/Polymères
bordeaux.journalBiochimica et Biophysica Acta:Biomembranes
bordeaux.page287-299
bordeaux.volume1838
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue1 PART B
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01368935
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01368935v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biochimica%20et%20Biophysica%20Acta:Biomembranes&rft.date=2014&rft.volume=1838&rft.issue=1%20PART%20B&rft.spage=287-299&rft.epage=287-299&rft.eissn=0005-2736&rft.issn=0005-2736&rft.au=BERTHELOT,%20Karine&LECOMTE,%20Sophie&ESTEVEZ,%20Yannick&ZHENDRE,%20Vanessa&HENRY,%20Sarah&rft.genre=article


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