Mostrar el registro sencillo del ítem

Candida albicans PPR proteins are required for the expression of respiratory Complex I subunits.

dc.rights.licenseopenen_US
dc.contributor.authorWENDA, Joanna Maria
dc.contributor.authorDRZEWICKA, Katarzyna
dc.contributor.authorMULICA, Patrycja
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorTETAUD, Emmanuel
IDREF: 25436697X
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorDI RAGO, Jean Paul
dc.contributor.authorGOLIK, Paweł
dc.contributor.authorŁABĘDZKA-DMOCH, Karolina
dc.date.accessioned2024-10-01T09:26:42Z
dc.date.available2024-10-01T09:26:42Z
dc.date.issued2024-07-29
dc.identifier.issn1943-2631en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/202082
dc.description.abstractEnPentatricopeptide (PPR) proteins bind RNA and are present in mitochondria and chloroplasts of Eukaryota. In fungi they are responsible for controlling mitochondrial genome expression, mainly on the posttranscriptional level. Candida albicans is a human opportunistic pathogen with a facultative anaerobic metabolism which, unlike the model yeast S. cerevisiae, possesses mitochondrially encoded respiratory Complex I (CI) subunits and does not tolerate loss of mtDNA. We characterized the function of 4 PPR proteins of C. albicans that lack orthologs in S. cerevisiae, and found that they are required for the expression of mitochondrially-encoded CI subunits. We demonstrated that these proteins localize to mitochondria and are essential to maintain the respiratory capacity of cells. Deletion of genes encoding these PPR proteins results in changes in steady state levels of mitochondrial RNAs and proteins. We demonstrated that C. albicans cells lacking CaPpr4, CaPpr11, and CaPpr13 proteins show no CI assembly, whereas the lack of CaPpr7p results in a decreased CI activity. CaPpr13p is required to maintain the bicistronic NAD4L-NAD5 mRNA, whereas the other three PPR proteins are likely involved in translation-related assembly of mitochondrially encoded CI subunits. In addition, we show that CaAep3p which is an ortholog of ScAep3p, performs the evolutionary conserved function of controlling expression of the ATP8-ATP6 mRNA. We also show that C. albicans cells lacking PPR proteins express a higher level of the inducible alternative oxidase (AOX2) which likely rescues respiratory defects and compensates for oxidative stress.
dc.language.isoENen_US
dc.title.enCandida albicans PPR proteins are required for the expression of respiratory Complex I subunits.
dc.title.alternativeGeneticsen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1093/genetics/iyae124en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed39073444en_US
bordeaux.journalGeneticsen_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.identifierhal-04716018
hal.version1
hal.date.transferred2024-10-01T09:26:45Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Genetics&rft.date=2024-07-29&rft.eissn=1943-2631&rft.issn=1943-2631&rft.au=WENDA,%20Joanna%20Maria&DRZEWICKA,%20Katarzyna&MULICA,%20Patrycja&TETAUD,%20Emmanuel&DI%20RAGO,%20Jean%20Paul&rft.genre=article


Archivos en el ítem

ArchivosTamañoFormatoVer

No hay archivos asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem