Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ASENCIO, Corinne | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | HERVÉ, Perrine | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MORAND, Pauline | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | OLIVERES, Quentin | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MOREL, Chloé Alexandra | |
| hal.structure.identifier | TBM-Core [Bordeaux] [UMS3427 - INSERM US005] | |
| dc.contributor.author | PROUZET-MAULEON, Valérie | |
| hal.structure.identifier | Centre de résonance magnétique des systèmes biologiques [CRMSB] | |
| dc.contributor.author | BIRAN, Marc | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | MONIC, Sarah | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BONHIVERS, Mélanie | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | ROBINSON, Derrick Roy | |
| dc.contributor.author | OUELLETTE, Marc | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | RIVIÈRE, Loïc | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | BRINGAUD, Frédéric | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | TETAUD, Emmanuel | |
| dc.date.accessioned | 2024-10-01T09:16:05Z | |
| dc.date.available | 2024-10-01T09:16:05Z | |
| dc.date.issued | 2024-06-01 | |
| dc.identifier.issn | 1365-2958 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/202081 | |
| dc.description.abstractEn | Kinetoplastids are unicellular eukaryotic flagellated parasites found in a wide range of hosts within the animal and plant kingdoms. They are known to be responsible in humans for African sleeping sickness (Trypanosoma brucei), Chagas disease (Trypanosoma cruzi), and various forms of leishmaniasis (Leishmania spp.), as well as several animal diseases with important economic impact (African trypanosomes, including Trypanosoma congolense). Understanding the biology of these parasites necessarily implies the ability to manipulate their genomes. In this study, we demonstrate that transfection of a ribonucleoprotein complex, composed of recombinant Streptococcus pyogenes Cas9 (SpCas9) and an in vitro-synthesized guide RNA, results in rapid and efficient genetic modifications of trypanosomatids, in marker-free conditions. This approach was successfully developed to inactivate, delete, and mutate candidate genes in various stages of the life cycle of T. brucei and T. congolense, and Leishmania promastigotes. The functionality of SpCas9 in these parasites now provides, to the research community working on these parasites, a rapid and efficient method of genome editing, without requiring plasmid construction and selection by antibiotics but requires only cloning and PCR screening of the clones. Importantly, this approach is adaptable to any wild-type parasite. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Gene Editing | |
| dc.subject.en | CRISPR-Cas Systems | |
| dc.subject.en | Ribonucleoproteins | |
| dc.subject.en | Streptococcus pyogenes | |
| dc.subject.en | Leishmania | |
| dc.subject.en | CRISPR-Associated Protein 9 | |
| dc.subject.en | RNA | |
| dc.subject.en | Guide | |
| dc.subject.en | CRISPR-Cas Systems | |
| dc.subject.en | Trypanosoma brucei brucei | |
| dc.subject.en | Trypanosoma | |
| dc.subject.en | Transfection | |
| dc.title.en | Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania. | |
| dc.title.alternative | Mol Microbiol | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1111/mmi.15256 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Microbiologie et Parasitologie | en_US |
| dc.identifier.pubmed | 38558208 | en_US |
| bordeaux.journal | Molecular Microbiology | en_US |
| bordeaux.page | 1079-1094 | en_US |
| bordeaux.volume | 121 | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.issue | 6 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Microbiology&rft.date=2024-06-01&rft.volume=121&rft.issue=6&rft.spage=1079-1094&rft.epage=1079-1094&rft.eissn=1365-2958&rft.issn=1365-2958&rft.au=ASENCIO,%20Corinne&HERV%C3%89,%20Perrine&MORAND,%20Pauline&OLIVERES,%20Quentin&MOREL,%20Chlo%C3%A9%20Alexandra&rft.genre=article |
