Purification process of recombinant monoclonal antibodies with mixed mode chromatography
| dc.rights.license | open | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | MARIA, Sophie | |
| dc.contributor.author | JOUCLA, Gilles | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | GARBAY, Bertrand
IDREF: 033777551 | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | DIERYCK, Wilfrid | |
| hal.structure.identifier | Univ Bordeaux, Ctr Genom Fonct, Plateforme Protéome | |
| dc.contributor.author | LOMENECH, Anne Marie | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | SANTARELLI, Xavier | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | CABANNE, Charlotte | |
| dc.date.accessioned | 2020 | |
| dc.date.available | 2020 | |
| dc.date.issued | 2015 | |
| dc.identifier.issn | 0021-9673 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/20189 | |
| dc.description.abstractEn | An innovative process to purify mAb from CHO cell culture supernatant was developed. This three-step process involved two mixed mode resins and an anion exchange membrane. We used a human IgG mixture to determine the optimal conditions for each purification step. Thereafter, the whole process was evaluated and improved for the purification of a recombinant mAb produced in the supernatant of CHO cells. Once optimized, yield and purity of 88% and 99.9%, respectively were comparable to those obtained in a conventional process based on a capture step using protein A. In addition, aggregates, HCPs and DNA levels in the purified fraction were below regulatory specifications. Then we used mass spectrometry to identify contaminating proteins in the antibody fraction in order to highlight the behavior of HCPs. (C) 2015 Elsevier B.V. All rights reserved. | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.subject.en | ADSORBENTS | |
| dc.subject.en | IDENTIFICATION | |
| dc.subject.en | AGGREGATION | |
| dc.subject.en | Mixed mode chromatography | |
| dc.subject.en | Antibody | |
| dc.subject.en | Host cell proteins | |
| dc.subject.en | Purification process | |
| dc.subject.en | CHARGE INDUCTION CHROMATOGRAPHY | |
| dc.subject.en | HOST-CELL PROTEINS | |
| dc.subject.en | SEPARATION | |
| dc.subject.en | CAPTURE | |
| dc.subject.en | BINDING | |
| dc.title.en | Purification process of recombinant monoclonal antibodies with mixed mode chromatography | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.chroma.2015.03.018 | |
| dc.subject.hal | Chimie/Polymères | |
| bordeaux.journal | Journal of Chromatography A | |
| bordeaux.page | 57-64 | |
| bordeaux.volume | 1393 | |
| bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-01373233 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-01373233v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Chromatography%20A&rft.date=2015&rft.volume=1393&rft.spage=57-64&rft.epage=57-64&rft.eissn=0021-9673&rft.issn=0021-9673&rft.au=MARIA,%20Sophie&JOUCLA,%20Gilles&GARBAY,%20Bertrand&DIERYCK,%20Wilfrid&LOMENECH,%20Anne%20Marie&rft.genre=article |
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