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Enrichment of Large Numbers of Splenic Mouse Dendritic Cells After Injection of Flt3L-Producing Tumor Cells

dc.rights.licenseopenen_US
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorSANTA, Pauline
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorROUBERTIE, Anaïs
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorLOIZON, Severine
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorGARREAU, Anne
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorFERRIERE, Amandine
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorDULUC, Dorothee
hal.structure.identifierImmunology from Concept and Experiments to Translation = Immunologie Conceptuelle, Expérimentale et Translationnelle [ImmunoConcept]
dc.contributor.authorSISIRAK, Vanja
dc.date.accessioned2024-09-17T09:18:20Z
dc.date.available2024-09-17T09:18:20Z
dc.date.issued2023-03
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/201619
dc.description.abstractEnDendritic cells (DCs) are antigen-presenting cells (APCs) that shape innate and adaptive immunity. There are multiple subsets of DCs distinguished according to their phenotype and functional specialization. DCs are present in lymphoid organs and across multiple tissues. However, their frequency and numbers at these sites are very low making their functional study difficult. Multiple protocols have been developed to generate DCs in vitro from bone marrow progenitors, but they do not fully recapitulate DC complexity found in vivo. Therefore, directly amplifying endogenous DCs in vivo appears as an option to overcome this specific caveat. In this chapter, we describe a protocol to amplify murine DCs in vivo by the injection of a B16 melanoma cell line expressing the trophic factor FMS-like tyrosine kinase 3 ligand (Flt3L). We have also compared two methods of magnetic sorting of amplified DCs, both giving high yields of total murine DCs, but different representation of the main DC subsets found in vivo. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
dc.language.isoENen_US
dc.source.titleDendritric cellsen_US
dc.subject.enConventional dendritic cells
dc.subject.enDC enrichment
dc.subject.enFlt3L
dc.subject.enPlasmacytoid dendritic cells
dc.title.enEnrichment of Large Numbers of Splenic Mouse Dendritic Cells After Injection of Flt3L-Producing Tumor Cells
dc.typeChapitre d'ouvrageen_US
dc.identifier.doi10.1007/978-1-0716-2938-3_13en_US
dc.subject.halSciences du Vivant [q-bio]/Immunologieen_US
dc.identifier.pubmed36905517en_US
bordeaux.page173-186en_US
bordeaux.volume2618en_US
bordeaux.hal.laboratoriesImmunoConcEpT - UMR 5164en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.inpressnonen_US
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=Dendritric%20cells&rft.date=2023-03&rft.volume=2618&rft.spage=173-186&rft.epage=173-186&rft.au=SANTA,%20Pauline&ROUBERTIE,%20Ana%C3%AFs&LOIZON,%20Severine&GARREAU,%20Anne&FERRIERE,%20Amandine&rft.genre=unknown


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