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AtALMT5 mediates vacuolar fumarate import and regulates the malate/fumarate balance in Arabidopsis

hal.structure.identifierInstitut des Sciences des Plantes de Montpellier [IPSIM]
dc.contributor.authorDOIREAU, Roxane
hal.structure.identifierInstitut des Sciences des Plantes de Montpellier [IPSIM]
dc.contributor.authorJAŚLAN, Justyna
hal.structure.identifierInstitut des Sciences des Plantes de Montpellier [IPSIM]
dc.contributor.authorCUBERO-FONT, Paloma
hal.structure.identifierInstitut des Sciences des Plantes de Montpellier [IPSIM]
dc.contributor.authorDEMES-CAUSSE, Elsa
hal.structure.identifierInstitut des Sciences des Plantes de Montpellier [IPSIM]
dc.contributor.authorBERTAUX, Karen
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCASSAN, Cédric
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierMetaboHUB
dc.contributor.authorPÉTRIACQ, Pierre
hal.structure.identifierInstitut des Sciences des Plantes de Montpellier [IPSIM]
dc.contributor.authorDE ANGELI, Alexis
dc.date.issued2024
dc.identifier.issn0028-646X
dc.description.abstractEnMalate and fumarate constitute a significant fraction of the carbon fixed by photosynthesis, and they are at the crossroad of central metabolic pathways. In Arabidopsis thaliana, they are transiently stored in the vacuole to keep cytosolic homeostasis. The malate and fumarate transport systems of the vacuolar membrane are key players in the control of cell metabolism. Notably, the molecular identity of these transport systems remains mostly unresolved. We used a combination of imaging, electrophysiology and molecular physiology to identify an important molecular actor of dicarboxylic acid transport across the tonoplast. Here, we report the function of the A. thaliana Aluminium-Activated Malate Transporter 5 (AtALMT5). We characterised its ionic transport properties, expression pattern, localisation and function in vivo. We show that AtALMT5 is expressed in photosynthetically active tissues and localised in the tonoplast. Patch-clamp and in planta analyses demonstrated that AtALMT5 is an ion channel-mediating fumarate loading of the vacuole. We found in almt5 plants a reduced accumulation of fumarate in the leaves, in parallel with increased malate concentrations. These results identified AtALMT5 as an ion channel-mediating fumarate transport in the vacuoles of mesophyll cells and regulating the malate/fumarate balance in Arabidopsis.
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enArabidopsis thaliana
dc.subject.encarbon sink
dc.subject.enion transporter
dc.subject.enorganic acids
dc.subject.enstomata
dc.subject.envacuole
dc.title.enAtALMT5 mediates vacuolar fumarate import and regulates the malate/fumarate balance in Arabidopsis
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.20077
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalNew Phytologist
bordeaux.page811-824
bordeaux.volume244
bordeaux.issue3
bordeaux.peerReviewedoui
hal.identifierhal-04690577
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04690577v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2024&rft.volume=244&rft.issue=3&rft.spage=811-824&rft.epage=811-824&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=DOIREAU,%20Roxane&JA%C5%9ALAN,%20Justyna&CUBERO-FONT,%20Paloma&DEMES-CAUSSE,%20Elsa&BERTAUX,%20Karen&rft.genre=article


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