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Rubber particle proteins REF1 and SRPP1 interact differently with native lipids extracted from Hevea brasiliensis latex

dc.rights.licenseopen
hal.structure.identifierDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University
dc.contributor.authorWADEESIRISAK, Kanthida
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCASTANO, Sabine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorBERTHELOT, Karine
hal.structure.identifierIngénierie des Agro-polymères et Technologies Émergentes [UMR IATE]
dc.contributor.authorVAYSSE, Laurent
hal.structure.identifierIngénierie des Agro-polymères et Technologies Émergentes [UMR IATE]
dc.contributor.authorBONFILS, Frédéric
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorPERUCH, Frédéric
IDREF: 152900748
hal.structure.identifierDepartment of Biotechnology, Faculty of Agro-Industry, Kasetsart University
dc.contributor.authorRATTANAPORN, Kittipong
dc.contributor.authorLIENGPRAYOON, Siriluck
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLECOMTE, Sophie
hal.structure.identifierIngénierie des Agro-polymères et Technologies Émergentes [UMR IATE]
dc.contributor.authorBOTTIER, Céline
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2017
dc.identifier.issn0005-2736
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20136
dc.description.abstractEnRubber particle membranes from the Hevea latex contain predominantly two proteins, REF1 and SRPP1 involved in poly(cis-1,4-isoprene) synthesis or rubber quality. The repartition of both proteins on the small or large rubber particles seems to differ, but their role in the irreversible coagulation of the rubber particle is still unknown. In this study we highlighted the different modes of interactions of both recombinant proteins with different classes of lipids extracted from Hevea brasiliensis latex, and defined as phospholipids (PL), glycolipids (GL) and neutral lipids (NL). We combined two biophysical methods, polarization modulated-infrared reflection adsorption spectroscopy (PM-IRRAS) and ellipsometry to elucidate their interactions with monolayers of each class of lipids. REF1 and SRPP1 interactions with native lipids are clearly different; SRPP1 interacts mostly in surface with PL, GL or NL, without modification of its structure. In contrast REF1 inserts deeply in the lipid monolayers with all lipid classes. With NL, REF1 is even able to switch from α-helice conformation to β-sheet structure, as in its aggregated form (amyloid form). Interaction between REF1 and NL may therefore have a specific role in the irreversible coagulation of rubber particles
dc.description.sponsorshipANR-10-CD2I-08
dc.description.sponsorshipDe la particule de caoutchouc d'hévéa à la structure et aux propriétés du caoutchouc naturel: vers l'optimisation des performances du caoutchouc naturel - ANR-14-CE07-0026
dc.language.isoen
dc.publisherElsevier
dc.subject.enHevea brasiliensis rubber particle proteins
dc.subject.enLangmuir monolayer
dc.subject.enLatex native lipids
dc.subject.enPolarization modulated-infrared reflection absorption spectroscopy
dc.subject.enRubber elongation factor
dc.subject.enSmall rubber particle protein
dc.title.enRubber particle proteins REF1 and SRPP1 interact differently with native lipids extracted from Hevea brasiliensis latex
dc.typeArticle de revue
dc.identifier.doi10.1016/j.bbamem.2016.11.010
dc.subject.halChimie/Polymères
dc.subject.halChimie/Matériaux
bordeaux.journalBiochimica et Biophysica Acta:Biomembranes
bordeaux.page201-210
bordeaux.volume1859
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue2
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01473711
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01473711v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biochimica%20et%20Biophysica%20Acta:Biomembranes&rft.date=2017&rft.volume=1859&rft.issue=2&rft.spage=201-210&rft.epage=201-210&rft.eissn=0005-2736&rft.issn=0005-2736&rft.au=WADEESIRISAK,%20Kanthida&CASTANO,%20Sabine&BERTHELOT,%20Karine&VAYSSE,%20Laurent&BONFILS,%20Fr%C3%A9d%C3%A9ric&rft.genre=article


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