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hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorDERRICHE, M’hamed
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorNOUVEL, Laurent-Xavier
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorSAGNÉ, Eveline
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorSIMON, Elisa
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorROBERT, Hortensia
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorPOT, Gwendoline
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorGAUDINO, Maria
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorMEYER, Gilles
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorARFI, Yonathan
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorCITTI, Christine
hal.structure.identifierInteractions hôtes-agents pathogènes [Toulouse] [IHAP]
dc.contributor.authorBARANOWSKI, Eric
dc.date.accessioned2024-07-20T02:05:26Z
dc.date.available2024-07-20T02:05:26Z
dc.date.conference2024-07-07
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/201050
dc.description.abstractEnAdvances in understanding ICE horizontal transfer in ruminant mycoplasmas have been facilitated by the establishment of axenic, optimal conditions. However, these provided only limited information on the influence of host factors on this event which is known to play an important role in chromosomal transfer. To address this issue, ICE-transfer frequencies were analyzed in M. agalactiae under replicative environments of increasing complexity, from axenic and cell culture growth conditions to ex vivo infections using Precision-Cut Lung Slices (PCLS). Remarkably, co-incubation of mating partners with epithelial cells resulted in increasing ICE-transfer frequency by ca 200-fold when compared to axenic, optimized mating conditions. ICEs are known to integrate randomly in the mycoplasma chromosome and a similar number of different integration sites were found in axenic and cell conditions by whole-genome sequencing of transconjugants, thus ruling out the amplification of a few individual transconjugants with high fitness values in cell culture conditions. The positive effect of host-related environmental factors on the conjugative behavior of M. agalactiae has also been observed upon ex vivo infections of bovine PCLS. As expected, only sporadic transconjugants could be selected when mating partners were incubated alone in the culture medium without host cells. This study reveals that the conjugative properties of mycoplasma cells may be influenced by their replicative environment, with enhanced mating frequency upon co-incubation with host cells. Most importantly, these results also suggest that the frequency of horizontal gene transfer in vivo is likely to be underestimated.
dc.description.sponsorshipApproche rationnelle d'un vaccin Mycoplasma bovis - ANR-21-CE35-0008
dc.language.isoen
dc.title.enHost Environmental Factors Promote Horizontal Dissemination of Integrative Conjugative Elements (ICE) in Ruminant Mycoplasmas
dc.typeCommunication dans un congrès
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie
bordeaux.hal.laboratoriesBiologie du Fruit & Pathologie (BFP) - UMR 1332*
bordeaux.institutionUniversité de Bordeaux
bordeaux.institutionINRAE
bordeaux.conference.title25th Congres of the International Organization for Mycoplasmology
bordeaux.countryES
bordeaux.conference.cityLas Palmas De Gran Canaria
bordeaux.peerReviewedoui
hal.identifierhal-04653941
hal.version1
hal.invitednon
hal.proceedingsnon
hal.conference.end2024-07-11
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04653941v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=DERRICHE,%20M%E2%80%99hamed&NOUVEL,%20Laurent-Xavier&SAGN%C3%89,%20Eveline&SIMON,%20Elisa&ROBERT,%20Hortensia&rft.genre=unknown


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