Multiple deletions of candidate effector genes lead to the breakdown of partial grapevine resistance to downy mildew
hal.structure.identifier | Santé et agroécologie du vignoble [UMR SAVE] | |
hal.structure.identifier | University of California [Davis] [UC Davis] | |
dc.contributor.author | PAINEAU, Manon | |
hal.structure.identifier | University of California [Davis] [UC Davis] | |
dc.contributor.author | MINIO, Andrea | |
hal.structure.identifier | Santé de la vigne et qualité du vin [SVQV] | |
dc.contributor.author | MESTRE, Pere | |
hal.structure.identifier | Santé et agroécologie du vignoble [UMR SAVE] | |
dc.contributor.author | FABRE, Frédéric | |
hal.structure.identifier | Santé et agroécologie du vignoble [UMR SAVE] | |
dc.contributor.author | MAZET, Isabelle | |
hal.structure.identifier | Santé et agroécologie du vignoble [UMR SAVE] | |
dc.contributor.author | COUTURE, Carole | |
hal.structure.identifier | Institut de Génétique, Environnement et Protection des Plantes [IGEPP] | |
hal.structure.identifier | Institut National de Recherche en Informatique et en Automatique [Inria] | |
dc.contributor.author | LEGEAI, Fabrice | |
hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
dc.contributor.author | DUMARTINET, Thomas | |
hal.structure.identifier | University of California [Davis] [UC Davis] | |
dc.contributor.author | CANTU, Dario | |
hal.structure.identifier | Santé et agroécologie du vignoble [UMR SAVE] | |
dc.contributor.author | DELMOTTE, François | |
dc.date.issued | 2024-06-21 | |
dc.identifier.issn | 0028-646X | |
dc.description.abstractEn | Grapevine downy mildew, caused by the oomycete Plasmopara viticola (P. viticola, Berk. & M. A. Curtis; Berl. & De Toni), is a global threat to Eurasian wine grapes Vitis vinifera. Although resistant grapevine varieties are becoming more accessible, P. viticola populations are rapidly evolving to overcome these resistances. We aimed to uncover avirulence genes related to Rpv3.1-mediated grapevine resistance. We sequenced the genomes and characterized the development of 136 P. viticola strains on resistant and sensitive grapevine cultivars. A genome-wide association study was conducted to identify genomic variations associated with resistant-breaking phenotypes. We identified a genomic region associated with the breakdown of Rpv3.1 grapevine resistance (avrRpv3.1 locus). A diploid-aware reassembly of the P. viticola INRA-Pv221 genome revealed structural variations in this locus, including a 30 kbp deletion. Virulent P. viticola strains displayed multiple deletions on both haplotypes at the avrRpv3.1 locus. These deletions involve two paralog genes coding for proteins with 800-900 amino acids and signal peptides. These proteins exhibited a structure featuring LWY-fold structural modules, common among oomycete effectors. When transiently expressed, these proteins induced cell death in grapevines carrying Rpv3.1 resistance, confirming their avirulence nature. This discovery sheds light on the genetic mechanisms enabling P. viticola to adapt to grapevine resistance, laying a foundation for developing strategies to manage this destructive crop pathogen. | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/ | |
dc.subject.en | avirulence | |
dc.subject.en | effector | |
dc.subject.en | GWAS | |
dc.subject.en | oomycete | |
dc.subject.en | Plasmopara viticola (grapevine downy mildew) | |
dc.subject.en | resistance breakdown | |
dc.subject.en | structural variant | |
dc.title.en | Multiple deletions of candidate effector genes lead to the breakdown of partial grapevine resistance to downy mildew | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1111/nph.19861 | |
dc.subject.hal | Sciences de l'environnement | |
bordeaux.journal | New Phytologist | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-04633322 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-04633322v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2024-06-21&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=PAINEAU,%20Manon&MINIO,%20Andrea&MESTRE,%20Pere&FABRE,%20Fr%C3%A9d%C3%A9ric&MAZET,%20Isabelle&rft.genre=article |
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