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Zebrafish genome instability after exposure to model genotoxicants

dc.rights.licenseopenen_US
dc.contributor.authorŠRUT, Maja
dc.contributor.authorŠTAMBUK, Anamaria
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.contributor.authorKLOBUČAR, Goran I. V.
dc.date.accessioned2024-06-17T11:40:38Z
dc.date.available2024-06-17T11:40:38Z
dc.date.issued2015-02-22
dc.identifier.issn0963-9292en_US
dc.identifier.urihttps://www.researchgate.net/publication/272237676_Zebrafish_genome_instability_after_exposure_to_model_genotoxicants
dc.identifier.urioai:researchgate.net:272237676
dc.identifier.urioai:crossref.org:10.1007/s10646-015-1432-x
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/200497
dc.description.abstractEnSublethal exposure to environmental genotoxicants may impact genome integrity in affected organisms. It is therefore necessary to develop tools to measure the extent and longevity of genotoxicant-induced DNA damage, and choose appropriate model organisms for biomonitoring. To this end, markers of DNA damage were measured in zebrafish larvae and adults following exposure to model genotoxicants (benzo[a]pyrene and ethyl methanesulfonate). Specifically, we assessed primary DNA damage and the existence of potentially persistent genomic alterations through application of the comet assay, quantitative random amplified polymorphic DNA (qRAPD) and amplified fragment length polymorphism (AFLP) assays. Furthermore, expression of genes involved in DNA repair, oxidative stress response and xenobiotic metabolism was evaluated as well. Additionally, the AFLP method was applied to adult specimens 1 year after larval exposure to the genotoxicants to evaluate the longevity of the observed DNA alterations. Large numbers of DNA alterations were detected in larval DNA using the comet assay, qRAPD and AFLP, demonstrating that zebrafish larvae are a sensitive model for revealing genotoxic effects. Furthermore, some of these genomic alterations persisted into adulthood, indicating the formation of stable genomic modifications. qRAPD and AFLP methods proved to be highly sensitive to genotoxic effects, even in cases when the comet assay indicated a lack of significant damage. These results thus support the use of zebrafish larvae as a sensitive model for monitoring the impact of genotoxic insult and give evidence of the longevity of genomic modifications induced by genotoxic agents.
dc.language.isoENen_US
dc.sourceresearchgate
dc.sourcecrossref
dc.title.enZebrafish genome instability after exposure to model genotoxicants
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s10646-015-1432-xen_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalEcotoxicologyen_US
bordeaux.page887-902en_US
bordeaux.volume24en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue4en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04614248
hal.version1
hal.date.transferred2024-06-17T11:40:39Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Ecotoxicology&rft.date=2015-02-22&rft.volume=24&rft.issue=4&rft.spage=887-902&rft.epage=887-902&rft.eissn=0963-9292&rft.issn=0963-9292&rft.au=%C5%A0RUT,%20Maja&%C5%A0TAMBUK,%20Anamaria&BOURDINEAUD,%20Jean-Paul&KLOBU%C4%8CAR,%20Goran%20I.%20V.&rft.genre=article


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