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Genomic and gene expression responses to genotoxic stress in PAC2 zebrafish embryonic cell line

dc.rights.licenseopenen_US
dc.contributor.authorŠRUT, Maja
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.contributor.authorŠTAMBUK, Anamaria
dc.contributor.authorKLOBUČAR, Goran I. V.
dc.date.accessioned2024-06-17T11:37:54Z
dc.date.available2024-06-17T11:37:54Z
dc.date.issued2015-01-21
dc.identifier.issn0260-437Xen_US
dc.identifier.urihttps://www.researchgate.net/publication/269320189_Genomic_and_gene_expression_responses_to_genotoxic_stress_in_PAC2_zebrafish_embryonic_cell_line
dc.identifier.urioai:crossref.org:10.1002/jat.3113
dc.identifier.urioai:researchgate.net:269320189
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/200496
dc.description.abstractEnPAC2 cell line is, along most of the developed zebrafish cell lines, poorly characterized concerning its response to genotoxicants. To define the PAC2 cell line response to different forms of genotoxic stress, we exposed the cells to model genotoxic agents (benzo[a]pyrene, B[a]P, and ethyl methanesulfonate) and subsequently monitored DNA damage and alterations by using the battery of tests, including the Comet assay, quantitative random-amplified polymorphic DNA and amplified fragment length polymorphism. The expression of several DNA repair (xpc, xpd, hr23b, rad51, msh2) and oxidative stress response (sod (Cu/Zn)) genes was monitored as well. To obtain an indication of the PAC2 cell line metabolizing capacity, the expression of genes belonging to cyp1, cyp2 and cyp3 families was assessed upon exposure to B[a]P. Genotoxic responses were observed in all the used methods, and quantitative random-amplified polymorphic DNA and amplified fragment length polymorphism proved to be more sensitive by revealing DNA alterations even when the Comet assay indicated lack of significant damage. The PAC2 cell line demonstrated basal and B[a]P-induced expression of several cyp genes, suggesting its ability to metabolize indirect acting xenobiotics to a certain point. Based on these results, PAC2 cells seem to be sensitive zebrafish in vitro model in the genotoxicity assessment of the direct acting genotoxicant; however, they are less sensitive toward the indirect acting genotoxicant due to their limited metabolizing properties.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourceresearchgate
dc.subject.enAFLP
dc.subject.enComet assay
dc.subject.enDNA repair genes
dc.subject.enquantitative RAPD
dc.subject.enxenobiotic metabolism
dc.title.enGenomic and gene expression responses to genotoxic stress in PAC2 zebrafish embryonic cell line
dc.typeArticle de revueen_US
dc.identifier.doi10.1002/jat.3113en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalJournal of Applied Toxicologyen_US
bordeaux.page1381-1389en_US
bordeaux.volume35en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue11en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04614246
hal.version1
hal.date.transferred2024-06-17T11:37:55Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
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