Moderate temperature elevation increase susceptibility of early-life stage of the Mediterranean mussel, Mytilus galloprovincialis to metal-induced genotoxicity.
| dc.rights.license | open | en_US |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | BOUKADIDA, Khouloud | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | CACHOT, Jérôme
ORCID: 0000-0002-1492-9684 IDREF: 19667672X | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | MORIN, Benedicte
IDREF: 178000892 | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | CLERANDEAU, Christelle | |
| dc.contributor.author | BANNI, Mohamed | |
| dc.date.accessioned | 2024-06-06T09:18:59Z | |
| dc.date.available | 2024-06-06T09:18:59Z | |
| dc.date.issued | 2019-05-01 | |
| dc.identifier.issn | 1879-1026 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/200303 | |
| dc.description.abstractEn | The present study aims to evaluate the effects of copper and silver alone or along with a moderate temperature increase on embryonic development, DNA integrity and target gene expression levels in early life stages of Mytilus galloprovincialis. For this purpose, upon fertilized embryos were exposed to a sub-lethal concentration of Cu (9.54 μg/L), Ag (2.55 μg/L) and to the mixture of the two metals (Cu (6.67 μg/L) + Ag (1.47 μg/L)) along with a temperature gradient (18, 20 and 22 °C). In all experiments, larvae were exposed to stressors for 48 h except for those designed to DNA damage analysis exposed only for 24 h (before shell formation).Our results showed a significant increase in the percentage of malformed D-larvae (p < 0.05) with increasing temperature and exposure to silver and copper alone or in a mixture. Moreover, metal toxicity increased significantly (p < 0.05) with the temperature rise. Genotoxicity was evaluated using classic and modified with Formamidopyrimidine DNA glycosylase (Fpg) Comet assay. Results suggest that co-exposure to metals and temperature significantly increased DNA damage on mussel larvae with a more accentuated oxidative damage. A significant transcription modulation was observed for genes involved in DNA repair and DNA replication (p53, DNA ligase II and topoisomerase II) when larvae are exposed to a single stressor. However, in the case of multiple stresses, caspase involved in the cell apoptosis pathway was overexpressed. Our study suggests that mussel larvae exposed to a moderate increase in temperature may have a compromised ability to defend against genotoxicity. This is particularly relevant in the context of global warming and thermal pollution. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Animals | |
| dc.subject.en | Apoptosis | |
| dc.subject.en | Comet Assay | |
| dc.subject.en | Copper | |
| dc.subject.en | DNA Repair | |
| dc.subject.en | Embryo | |
| dc.subject.en | Nonmammalian | |
| dc.subject.en | Embryonic Development | |
| dc.subject.en | Hot Temperature | |
| dc.subject.en | Larva | |
| dc.subject.en | Mytilus | |
| dc.subject.en | Real-Time Polymerase Chain Reaction | |
| dc.subject.en | Silver | |
| dc.title.en | Moderate temperature elevation increase susceptibility of early-life stage of the Mediterranean mussel, Mytilus galloprovincialis to metal-induced genotoxicity. | |
| dc.title.alternative | Sci Total Environ | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1016/j.scitotenv.2019.01.215 | en_US |
| dc.subject.hal | Sciences de l'environnement | en_US |
| dc.identifier.pubmed | 30716625 | en_US |
| bordeaux.journal | Science of the Total Environment | en_US |
| bordeaux.page | 351-360 | en_US |
| bordeaux.volume | 663 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.team | EA | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | pubmed | |
| hal.identifier | hal-04603156 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-06-06T09:19:01Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | pubmed | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Science%20of%20the%20Total%20Environment&rft.date=2019-05-01&rft.volume=663&rft.spage=351-360&rft.epage=351-360&rft.eissn=1879-1026&rft.issn=1879-1026&rft.au=BOUKADIDA,%20Khouloud&CACHOT,%20J%C3%A9r%C3%B4me&MORIN,%20Benedicte&CLERANDEAU,%20Christelle&BANNI,%20Mohamed&rft.genre=article |
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