Revealing the lipidome and proteome of Arabidopsis thaliana plasma membrane
| dc.rights.license | open | en_US |
| dc.contributor.author | BAHAMMOU, Delphine | |
| dc.contributor.author | RECORBET, Ghislaine | |
| dc.contributor.author | CASSIM, Adiilah Mamode | |
| dc.contributor.author | ROBERT, Franck | |
| dc.contributor.author | BALLIAU, Thierry | |
| dc.contributor.author | DELFT, Pierre Van | |
| dc.contributor.author | HADDAD, Youcef | |
| dc.contributor.author | MOUNIER, Arnaud | |
| dc.contributor.author | MONGRAND, Sébastien | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| hal.structure.identifier | Plateforme Bordeaux Metabolome | |
| dc.contributor.author | FOUILLEN, Laetitia
ORCID: 0000-0002-1204-9296 IDREF: 136936385 | |
| hal.structure.identifier | Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement [INRAE] | |
| dc.contributor.author | SIMON-PLAS, Françoise | |
| dc.date.accessioned | 2024-05-15T08:01:42Z | |
| dc.date.available | 2024-05-15T08:01:42Z | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199846 | |
| dc.description.abstractEn | Abstract The plant plasma membrane (PM) plays a key role in nutrition, cell homeostasis, perception of environmental signals, and set-up of appropriate adaptive responses. An exhaustive and quantitative description of the whole set of lipids and proteins constituting the PM is thus necessary to understand how its individual components, the way they are organized and interact together, allow to fulfill such essential physiological functions. Here we provide by state-of-the-art approaches the first combined reference of the plant PM lipidome and proteome from Arabidopsis thaliana suspension cell culture. We identified a core set of 2,165 proteins (406 of which had not been shown associated to PM previously), which is by far the largest set of available data concerning the plant PM proteome. Using the same samples, we combined lipidomic approaches, allowing the identification and quantification of an unprecedented repertoire of 405 molecular species of lipids. We showed that the different classes of lipids (sterols, phospholipids, and sphingolipids) were present in similar proportions in the plant PM. Within each lipid class, the precise amount of each lipid family and the relative proportion of each molecular species were then determined, allowing us to establish the complete lipidome of Arabidopsis PM, and highlighting specific characteristics of the different molecular species of lipids (for instance fatty acyl chain length and saturation according to the polar head). Results obtained are consistent with plant PM being an ordered mosaic of domains and point to a finely tuned adjustment of the molecular characteristics of lipids and proteins. More than a hundred proteins related to lipid metabolism, transport or signaling have been identified and put in perspective of the lipids with which they are associated. All these results provide an overall view of both the organization and the functioning of the PM. | |
| dc.description.sponsorship | Vers un modèle intégratif de la bicouche lipidique de la membrane plasmique végétale - ANR-19-CE20-0016 | en_US |
| dc.description.sponsorship | Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation | en_US |
| dc.language.iso | EN | en_US |
| dc.title.en | Revealing the lipidome and proteome of Arabidopsis thaliana plasma membrane | |
| dc.type | Document de travail - Pré-publication | en_US |
| dc.identifier.doi | 10.1101/2023.05.14.540643 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio] | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire | en_US |
| bordeaux.hal.laboratories | Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-04190430 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.subtype | Prepublication/Preprint | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=BAHAMMOU,%20Delphine&RECORBET,%20Ghislaine&CASSIM,%20Adiilah%20Mamode&ROBERT,%20Franck&BALLIAU,%20Thierry&rft.genre=preprint |
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