A global LC-MS 2 -based methodology to identify and quantify anionic phospholipids in plant samples
| dc.rights.license | open | en_US |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | GENVA, Manon | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | FOUGÈRE, Louise | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | BAHAMMOU, Delphine | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| hal.structure.identifier | Plateforme Bordeaux Metabolome | |
| dc.contributor.author | MONGRAND, Sébastien | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| hal.structure.identifier | Plateforme Bordeaux Metabolome | |
| dc.contributor.author | BOUTTÉ, Yohann | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| hal.structure.identifier | Plateforme Bordeaux Metabolome | |
| dc.contributor.author | FOUILLEN, Laetitia
ORCID: 0000-0002-1204-9296 IDREF: 136936385 | |
| dc.date.accessioned | 2024-05-15T07:53:18Z | |
| dc.date.available | 2024-05-15T07:53:18Z | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199845 | |
| dc.description.abstractEn | Anionic phospholipids (PS, PA, PI, PIPs) are low abundant phospholipids with impactful functions in cell signaling, membrane trafficking and cell differentiation processes. They can be quickly metabolized and can transiently accumulate at define spots within the cell or an organ to respond to physiological or environmental stimuli. As even a small change in their composition profile will produce a significant effect on biological processes, it is crucial to develop a sensitive and optimized analytical method to accurately detect and quantify them. While thin layer chromatography (TLC) separation coupled with gas chromatography (GC) detection methods already exist, they do not allow for precise, sensitive and accurate quantification of all anionic phospholipid species. Here we developed a method based on high performance liquid chromatography (HPLC) combined with two-dimensional mass spectrometry (MS 2 ) by MRM mode to detect and quantify all molecular species and classes of anionic phospholipids in one-shot. This method is based on a derivatization step by methylation that greatly enhances the ionization, the separation of each peaks, the peak resolution as well as the limit of detection and quantification for each individual molecular species, and more particularly for PA and PS. Our method universally works in various plant samples. Remarkably, we identified that PS is enriched with very long chain fatty acids in the roots but not in aerial organs of Arabidopsis thaliana . Our work thus paves the way to new studies on how the composition of anionic lipids is finely tuned during plant development and environmental responses. Significance Statement While anionic phospholipids have key functions in plant cellular processes, their low concentration in biological samples and their low stability during the analysis complicate their quantification. Here, we present the first one-shot analytical method for the profiling and quantification of all anionic phospholipid classes and species from plant tissues with unprecedented sensitivity. This method open the way to future studies requiring a fine quantification of anionic phospholipids to understand their role in plant cell processes. | |
| dc.description.sponsorship | Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010 | en_US |
| dc.description.sponsorship | Vers un modèle intégratif de la bicouche lipidique de la membrane plasmique végétale - ANR-19-CE20-0016 | en_US |
| dc.description.sponsorship | Mécanismes du pattern lipidique du réseau trans-Golgien (trans-Golgi Network) et rôles dans le tri des protéines, la polarité cellulaire et le développement des plantes - ANR-18-CE13-0025 | en_US |
| dc.language.iso | EN | en_US |
| dc.subject.en | Anionic phospholipids | |
| dc.subject.en | plant lipids | |
| dc.subject.en | tandem mass spectrometry | |
| dc.subject.en | methylation | |
| dc.subject.en | phosphoinositides | |
| dc.title.en | A global LC-MS 2 -based methodology to identify and quantify anionic phospholipids in plant samples | |
| dc.type | Document de travail - Pré-publication | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Botanique | en_US |
| dc.subject.hal | Chimie/Chimie analytique | en_US |
| bordeaux.hal.laboratories | Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-04266576 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.subtype | Prepublication/Preprint | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=GENVA,%20Manon&FOUG%C3%88RE,%20Louise&BAHAMMOU,%20Delphine&MONGRAND,%20S%C3%A9bastien&BOUTT%C3%89,%20Yohann&rft.genre=preprint |
