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Bcl-xL Is Spontaneously Inserted into Preassembled Nanodiscs and Stimulates Bax Insertion in a Cell-Free Protein Synthesis System

dc.rights.licenseopenen_US
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorROUCHIDANE EYITAYO, Akande
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorBOUDIER-LEMOSQUET, Axel
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCHAIGNEPAIN, Stephane
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorPRIAULT, Muriel
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorMANON, Stephen
dc.date.accessioned2024-05-02T08:09:40Z
dc.date.available2024-05-02T08:09:40Z
dc.date.issued2023
dc.identifier.issn2218-273Xen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199564
dc.description.abstractEnThe antiapoptotic protein Bcl-xL is a major regulator of cell death and survival, but many aspects of its functions remain elusive. It is mostly localized in the mitochondrial outer membrane (MOM) owing to its C-terminal hydrophobic α-helix. In order to gain further information about its membrane organization, we set up a model system combining cell-free protein synthesis and nanodisc insertion. We found that, contrary to its proapoptotic partner Bax, neosynthesized Bcl-xL was spontaneously inserted into nanodiscs. The deletion of the C-terminal α-helix of Bcl-xL prevented nanodisc insertion. We also found that nanodisc insertion protected Bcl-xL against the proteolysis of the 13 C-terminal residues that occurs during expression of Bcl-xL as a soluble protein in E. coli. Interestingly, we observed that Bcl-xL increased the insertion of Bax into nanodiscs, in a similar way to that which occurs in mitochondria. Cell-free synthesis in the presence of nanodiscs is, thus, a suitable model system to study the molecular aspects of the interaction between Bcl-xL and Bax during their membrane insertion.
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/
dc.subject.enapoptosis
dc.subject.enBcl-2 family
dc.subject.ennanodiscs
dc.title.enBcl-xL Is Spontaneously Inserted into Preassembled Nanodiscs and Stimulates Bax Insertion in a Cell-Free Protein Synthesis System
dc.typeArticle de revueen_US
dc.identifier.doi10.3390/biom13060876en_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaireen_US
bordeaux.journalBiomoleculesen_US
bordeaux.page876en_US
bordeaux.volume13en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.issue6en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-04255612
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomolecules&rft.date=2023&rft.volume=13&rft.issue=6&rft.spage=876&rft.epage=876&rft.eissn=2218-273X&rft.issn=2218-273X&rft.au=ROUCHIDANE%20EYITAYO,%20Akande&BOUDIER-LEMOSQUET,%20Axel&CHAIGNEPAIN,%20Stephane&PRIAULT,%20Muriel&MANON,%20Stephen&rft.genre=article


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