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Controlling differentiation of stem cells via bioactive disordered cues

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorZHANG, Yujie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorREMY, Murielle
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorAPARTSIN, Evgeny
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorPROUVE, Emilie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorFEUILLIE, Cecile
hal.structure.identifierPlateforme Aquitaine de Caractérisation des Matériaux [PLACAMAT]
dc.contributor.authorLABRUGERE, Christine
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCAM, Nithavong
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDURRIEU, Marie-Christine
dc.date.accessioned2024-04-30T12:56:07Z
dc.date.available2024-04-30T12:56:07Z
dc.date.issued2023
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199547
dc.description.abstractEnIdeal bone tissue engineering is to induce bone regeneration through the synergistic integration of biomaterial scaffolds, bone progenitor cells, and bone-forming factors. Biomimetic scaffolds imitate the native extracellular matrix (ECM) and are often utilized in vitro as analogues of the natural ECM to facilitate investigations of cell–ECM interactions and processes. In vivo, the cellular microenvironment has a crucial impact on regulating cell behavior and functions. A PET surface was activated and then functionalized with mimetic peptides to promote human mesenchymal stem cell (hMSC) adhesion and differentiation into an osteogenic lineage. Spray technology was used to randomly micropattern peptides (RGD and BMP-2 mimetic peptides) on the PET surface. The distribution of the peptides grafted on the surface, the roughness of the surfaces and the chemistry of the surfaces in each step of the treatment were ascertained by atomic force microscopy, fluorescence microscopy, time-of-flight secondary ion mass spectrometry, Toluidine Blue O assay, and X-ray photoelectron spectroscopy. Subsequently, cell lineage differentiation was evaluated by quantifying the expression of immunofluorescence markers: osteoblast markers (Runx-2, OPN) and osteocyte markers (E11, DMP1, and SOST). In this article, we hypothesized that a unique combination of bioactive micro/nanopatterns on a polymer surface improves the rate of morphology change and enhances hMSC differentiation. In DMEM, after 14 days, disordered micropatterned surfaces with RGD and BMP-2 led to a higher osteoblast marker expression than surfaces with a homogeneous dual peptide conjugation. Finally, hMSCs cultured in osteogenic differentiation medium (ODM) showed accelerated cell differentiation. In ODM, our results highlighted the expression of osteocyte markers when hMSCs were seeded on PET surfaces with random micropatterns.
dc.description.sponsorshipConception de surfaces bioinspirées avec des propriétés mécaniques et de bioactivité contrôlées pour la synthèse de plateforme in vitro de culture cellulaire - ANR-21-CE06-0031en_US
dc.language.isoENen_US
dc.title.enControlling differentiation of stem cells via bioactive disordered cues
dc.typeArticle de revueen_US
dc.identifier.doi10.1039/D3BM00605Ken_US
dc.subject.halChimie/Polymèresen_US
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale/Biomatériauxen_US
bordeaux.journalBiomaterials Scienceen_US
bordeaux.page6116-6134en_US
bordeaux.volume11en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.issue18en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-04243815
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomaterials%20Science&rft.date=2023&rft.volume=11&rft.issue=18&rft.spage=6116-6134&rft.epage=6116-6134&rft.au=ZHANG,%20Yujie&REMY,%20Murielle&APARTSIN,%20Evgeny&PROUVE,%20Emilie&FEUILLIE,%20Cecile&rft.genre=article


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