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Solid-state NMR molecular snapshots of Aspergillus fumigatus cell wall architecture during a conidial morphotype transition

dc.rights.licenseopenen_US
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLAMON, Gaelle
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLENDS, Alons
dc.contributor.authorVALSECCHI, Isabel
dc.contributor.authorWONG, Sarah Sze Wah
dc.contributor.authorDUPRES, Vincent
dc.contributor.authorLAFONT, Frank
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTOLCHARD, James
dc.contributor.authorSCHMITT, Christine
dc.contributor.authorMALLET, Adeline
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorGRELARD, Axelle
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
dc.contributor.authorMORVAN, Estelle
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDUFOURC, Erick
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorHABENSTEIN, Birgit
dc.contributor.authorGUIJARRO, J. Inaki
dc.contributor.authorAIMANIANDA, Vishukumar
hal.structure.identifierInstitut Européen de Chimie et Biologie [IECB]
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLOQUET, Antoine
dc.date.accessioned2024-04-24T09:59:30Z
dc.date.available2024-04-24T09:59:30Z
dc.date.issued2023-02-07
dc.identifier.issn0027-8424en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199303
dc.description.abstractEnWhile establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit through swollen and germinating stages, to form hyphae. During this morphotype transition, the conidial cell wall undergoes dynamic remodeling, which poses challenges to the host immune system and antifungal drugs. However, such cell wall reorganization during conidial germination has not been studied so far. Here, we explored the molecular rearrangement of Aspergillus fumigatus cell wall polysaccharides during different stages of germination. We took advantage of magic-angle spinning NMR to investigate the cell wall polysaccharides, without employing any destructive method for sample preparation. The breaking of dormancy was associated with a significant change in the molar ratio between the major polysaccharides β-1,3-glucan and α-1,3-glucan, while chitin remained equally abundant. The use of various polarization transfers allowed the detection of rigid and mobile polysaccharides; the appearance of mobile galactosaminogalactan was a molecular hallmark of germinating conidia. We also report for the first time highly abundant triglyceride lipids in the mobile matrix of conidial cell walls. Water to polysaccharides polarization transfers revealed an increased surface exposure of glucans during germination, while chitin remained embedded deeper in the cell wall, suggesting a molecular compensation mechanism to keep the cell wall rigidity. We complement the NMR analysis with confocal and atomic force microscopies to explore the role of melanin and RodA hydrophobin on the dormant conidial surface. Exemplified here using Aspergillus fumigatus as a model, our approach provides a powerful tool to decipher the molecular remodeling of fungal cell walls during their morphotype switching.
dc.description.sponsorshipIntegrative Biology of Emerging Infectious Diseasesen_US
dc.description.sponsorshipAmyloïdes fonctionnels formés par les hydrophobines du pathogène fongique Aspergillus fumigatus - ANR-16-CE11-0020en_US
dc.description.sponsorshipExplorer les polysaccharides fongiques de la paroi cellulaire pour les immunothérapiesen_US
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enAspergillus fumigatus
dc.subject.encell wall dynamics
dc.subject.enconidium
dc.subject.engermination
dc.subject.ensolid-state NMR
dc.subject.enAspergillus fumigatus
dc.title.enSolid-state NMR molecular snapshots of Aspergillus fumigatus cell wall architecture during a conidial morphotype transition
dc.typeArticle de revueen_US
dc.identifier.doi10.1073/pnas.2212003120en_US
dc.subject.halSciences du Vivant [q-bio]en_US
bordeaux.journalProceedings of the National Academy of Sciences of the United States of Americaen_US
bordeaux.volume120en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.issue6en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierpasteur-04124323
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20of%20the%20United%20States%20of%20America&rft.date=2023-02-07&rft.volume=120&rft.issue=6&rft.eissn=0027-8424&rft.issn=0027-8424&rft.au=LAMON,%20Gaelle&LENDS,%20Alons&VALSECCHI,%20Isabel&WONG,%20Sarah%20Sze%20Wah&DUPRES,%20Vincent&rft.genre=article


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