cDNA cloning and gene expression of ribosomal S9 protein gene in the mollusk Corbicula fluminea: A new potential biomarker of metal contamination up-regulated by cadmium and repressed by zinc
| dc.rights.license | open | en_US |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | ACHARD-JORIS, Maud | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | GONZALEZ, Patrice
ORCID: 0000-0003-2628-2993 IDREF: 14341917X | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | MARIE, Véronique | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | BAUDRIMONT, Magalie
IDREF: 105696722 | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | BOURDINEAUD, Jean-Paul | |
| dc.date.accessioned | 2024-04-15T15:05:36Z | |
| dc.date.available | 2024-04-15T15:05:36Z | |
| dc.date.issued | 2006-01-01 | |
| dc.identifier.issn | 0730-7268 | en_US |
| dc.identifier.uri | https://www.researchgate.net/publication/7259425_cDNA_cloning_and_gene_expression_of_ribosomal_S9_protein_gene_in_the_mollusk_Corbicula_fluminea_A_new_potential_biomarker_of_metal_contamination_up-regulated_by_cadmium_and_repressed_by_zinc | |
| dc.identifier.uri | oai:crossref.org:10.1897/05-211r.1 | |
| dc.identifier.uri | oai:researchgate.net:7259425 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199012 | |
| dc.description.abstractEn | Biological indicators can be used to assess polluted sites, but their success depends on their specificity. The aim of the present study was to identify a specific cadmium biomarker of the freshwater bivalve Corbicula fluminea. Differential display technique was used to identify new molecular indicators for cadmium exposure. Gene expression of C. fluminea transplanted into a cadmium- and zinc-contaminated river was compared to gene expression of bivalves from a reference site. One differentially expressed band was identified, and the entire cDNA sequence coding for the ribosomal protein S9 (rpS9) was cloned. The rpS9 gene expression was studied by real-time reverse transcription-polymerase chain reaction. After controlled cadmium (15 microg/L) and/or zinc (1 mg/L) laboratory contaminations of the bivalves, this gene was shown to be up-regulated by cadmium and down-regulated by zinc. A 48-d transplantation of animals into a polymetallic field contamination in the Lot river basin (Midi-Pyrénées region, France) showed that rpS9 gene regulation was correlated with the fluctuating cadmium and zinc water contents. To our knowledge, the existence of a gene that is up-regulated by cadmium and repressed by zinc is demonstrated here for the first time. | |
| dc.language.iso | EN | en_US |
| dc.source | crossref | |
| dc.source | researchgate | |
| dc.title.en | cDNA cloning and gene expression of ribosomal S9 protein gene in the mollusk Corbicula fluminea: A new potential biomarker of metal contamination up-regulated by cadmium and repressed by zinc | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1897/05-211r.1 | en_US |
| dc.subject.hal | Sciences de l'environnement | en_US |
| bordeaux.journal | Environmental Toxicology and Chemistry | en_US |
| bordeaux.page | 527 | en_US |
| bordeaux.volume | 25 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.issue | 2 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.team | EA | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | dissemin | |
| hal.identifier | hal-04547381 | |
| hal.version | 1 | |
| hal.date.transferred | 2024-04-15T15:05:38Z | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | true | |
| workflow.import.source | dissemin | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental%20Toxicology%20and%20Chemistry&rft.date=2006-01-01&rft.volume=25&rft.issue=2&rft.spage=527&rft.epage=527&rft.eissn=0730-7268&rft.issn=0730-7268&rft.au=ACHARD-JORIS,%20Maud&GONZALEZ,%20Patrice&MARIE,%20V%C3%A9ronique&BAUDRIMONT,%20Magalie&BOURDINEAUD,%20Jean-Paul&rft.genre=article |
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