ACHARD-JORIS, Maud; GONZALEZ, Patrice; MARIE, Véronique; BAUDRIMONT, Magalie; BOURDINEAUD, Jean-Paul

doi:10.1897/05-211r.1

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ACHARD-JORIS, Maud
Environnements et Paléoenvironnements OCéaniques [EPOC]

GONZALEZ, Patrice

Environnements et Paléoenvironnements OCéaniques [EPOC]

MARIE, Véronique
Environnements et Paléoenvironnements OCéaniques [EPOC]

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Environmental Toxicology and Chemistry. 2006-01-01, vol. 25, n° 2, p. 527

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Biological indicators can be used to assess polluted sites, but their success depends on their specificity. The aim of the present study was to identify a specific cadmium biomarker of the freshwater bivalve Corbicula fluminea. Differential display technique was used to identify new molecular indicators for cadmium exposure. Gene expression of C. fluminea transplanted into a cadmium- and zinc-contaminated river was compared to gene expression of bivalves from a reference site. One differentially expressed band was identified, and the entire cDNA sequence coding for the ribosomal protein S9 (rpS9) was cloned. The rpS9 gene expression was studied by real-time reverse transcription-polymerase chain reaction. After controlled cadmium (15 microg/L) and/or zinc (1 mg/L) laboratory contaminations of the bivalves, this gene was shown to be up-regulated by cadmium and down-regulated by zinc. A 48-d transplantation of animals into a polymetallic field contamination in the Lot river basin (Midi-Pyrénées region, France) showed that rpS9 gene regulation was correlated with the fluctuating cadmium and zinc water contents. To our knowledge, the existence of a gene that is up-regulated by cadmium and repressed by zinc is demonstrated here for the first time.< Réduire

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cDNA cloning and gene expression of ribosomal S9 protein gene in the mollusk Corbicula fluminea: A new potential biomarker of metal contamination up-regulated by cadmium and repressed by zinc

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