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Comparative Effects of Dietary Methylmercury on Gene Expression in Liver, Skeletal Muscle, and Brain of the Zebrafish ( Danio rerio )

dc.rights.licenseopenen_US
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorGONZALEZ, Patrice
ORCID: 0000-0003-2628-2993
IDREF: 14341917X
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDOMINIQUE, Yannick
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorMASSABUAU, Jean-Charles
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOUDOU, Alain
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorBOURDINEAUD, Jean-Paul
dc.date.accessioned2024-04-15T14:19:17Z
dc.date.available2024-04-15T14:19:17Z
dc.date.issued2005-06-01
dc.identifier.issn0013-936Xen_US
dc.identifier.urihttps://www.researchgate.net/publication/7758701_Comparative_Effects_of_Dietary_Methylmercury_on_Gene_Expression_in_Liver_Skeletal_Muscle_and_Brain_of_the_Zebrafish_Danio_rerio
dc.identifier.urioai:crossref.org:10.1021/es0483490
dc.identifier.urioai:researchgate.net:7758701
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199009
dc.description.abstractEnEffects of dietary methylmercury (MeHg) on gene expression were examined in three organs (liver, skeletal muscle, and brain) of the zebrafish (Danio rerio). Adult male fish were fed over 7, 21, and 63 days on three different diets: one control diet (C0: 0.08 microg of Hg g(-1), dry wt) and two diets (C1 and C2) contaminated by MeHg at 5 and 13.5 microg of Hg g(-1), dry wt. Total Hg and MeHg concentrations were determined in the three organs after each exposure duration, and a demethylation process was evidenced only in the liver. Thirteen genes known to be involved in antioxidant defenses, metal chelation, active efflux of organic compounds, mitochondrial metabolism, DNA repair, and apoptosis were investigated by quantitative real-time RT-PCR and normalized according to actin gene expression. Surprisingly, no change in the expression levels of these genes was observed in contaminated brain samples, although this organ accumulated the highest mercury concentration (63.5 +/- 4.4 microg g(-1), dry wt after 63 days). This lack of genetic response could explain the high neurotoxicity of MeHg. coxI and cytoplasmic and mitochondrial sod gene expressions were induced early in skeletal muscle and later in liver, indicating an impact on the mitochondrial metabolism and production of reactive oxygen species. Results demonstrated that skeletal muscle was not only an important storage reservoir but was also affected by MeHg contamination. The expression of the metallothionein mt2 and the DNA repair rad51 genes was up-regulated in liver between 21 and 63 days, whereas in skeletal muscle, mt2 remained uninduced, and gadd and rad51 were found to be repressed.
dc.language.isoENen_US
dc.sourcecrossref
dc.sourceresearchgate
dc.title.enComparative Effects of Dietary Methylmercury on Gene Expression in Liver, Skeletal Muscle, and Brain of the Zebrafish ( Danio rerio )
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/es0483490en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalEnvironmental Science and Technologyen_US
bordeaux.page3972-3980en_US
bordeaux.volume39en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue11en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamEAen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.identifierhal-04547247
hal.version1
hal.date.transferred2024-04-15T14:19:19Z
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exporttrue
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Environmental%20Science%20and%20Technology&rft.date=2005-06-01&rft.volume=39&rft.issue=11&rft.spage=3972-3980&rft.epage=3972-3980&rft.eissn=0013-936X&rft.issn=0013-936X&rft.au=GONZALEZ,%20Patrice&DOMINIQUE,%20Yannick&MASSABUAU,%20Jean-Charles&BOUDOU,%20Alain&BOURDINEAUD,%20Jean-Paul&rft.genre=article


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