An innovative and integrative assay for toxicity testing using individual fish embryos. Application to oxazepam
dc.rights.license | open | en_US |
hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
dc.contributor.author | GRANGER JOLY DE BOISSEL, Philippine | |
hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
dc.contributor.author | GONZALEZ, Patrice
ORCID: 0000-0003-2628-2993 IDREF: 14341917X | |
dc.contributor.author | BULETÉ, Audrey | |
hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
dc.contributor.author | DAFFE, Guillemine | |
hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
dc.contributor.author | CLERANDEAU, Christelle | |
dc.contributor.author | VULLIET, Emmanuelle | |
hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
dc.contributor.author | CACHOT, Jérôme
ORCID: 0000-0002-1492-9684 IDREF: 19667672X | |
dc.date.accessioned | 2024-04-15T12:38:03Z | |
dc.date.available | 2024-04-15T12:38:03Z | |
dc.date.issued | 2017-08-01 | |
dc.identifier.issn | 0045-6535 | en_US |
dc.identifier.uri | oai:crossref.org:10.1016/j.chemosphere.2017.04.067 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199005 | |
dc.description.abstractEn | This paper describes the development of an integrative embryo-toxicity assay in Japanese medaka allowing analysis of several toxicological endpoints together in a same individual. In this assay, embryos are topically exposed, and survival, hatching success, malformations, biometry, behaviour, and target gene expression are subsequently analysed in each individual. This assay was applied to oxazepam, an anxiolytic pharmaceutical compound currently found in wastewater treatment plant effluent. Even if oxazepam accumulation in embryos was very low, it caused spinal and cardiac malformations, delayed growth, erratic swimming and deregulation of genes involved in apoptosis, DNA repair and mitochondrial metabolism. Relationship between gene deregulation, abnormal behaviour, and developmental anomalies was demonstrated. This assay is sensitive enough to detect adverse effects at low chemical concentrations and at multiple endpoints in a unique fish embryo. This integrative embryo-toxicity assay is a powerful tool to characterize the spectrum of effects of new chemicals and also to link effects induced at different molecular, tissue and physiological levels. | |
dc.language.iso | EN | en_US |
dc.source | crossref | |
dc.subject.en | Anxiolytic pharmaceutical | |
dc.subject.en | Behaviour | |
dc.subject.en | Fish embryo-toxicity assay | |
dc.subject.en | Gene expression | |
dc.subject.en | Individual analyses | |
dc.subject.en | Malformations | |
dc.title.en | An innovative and integrative assay for toxicity testing using individual fish embryos. Application to oxazepam | |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.1016/j.chemosphere.2017.04.067 | en_US |
dc.subject.hal | Sciences de l'environnement | en_US |
bordeaux.journal | Chemosphere | en_US |
bordeaux.page | 468-477 | en_US |
bordeaux.volume | 181 | en_US |
bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.institution | CNRS | en_US |
bordeaux.team | EA | |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
bordeaux.import.source | dissemin | |
hal.popular | non | en_US |
hal.audience | Internationale | en_US |
hal.export | false | |
workflow.import.source | dissemin | |
dc.rights.cc | Pas de Licence CC | en_US |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Chemosphere&rft.date=2017-08-01&rft.volume=181&rft.spage=468-477&rft.epage=468-477&rft.eissn=0045-6535&rft.issn=0045-6535&rft.au=GRANGER%20JOLY%20DE%20BOISSEL,%20Philippine&GONZALEZ,%20Patrice&BULET%C3%89,%20Audrey&DAFFE,%20Guillemine&CLERANDEAU,%20Christelle&rft.genre=article |
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