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PDMS-based microfluidics for proteomic analysis.
hal.structure.identifier | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris [ESPCI Paris] | |
dc.contributor.author | DODGE, Arash | |
hal.structure.identifier | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris [ESPCI Paris] | |
hal.structure.identifier | Laboratoire de Physique Statistique de l'ENS [LPS] | |
hal.structure.identifier | Laboratoire Microfluidique, MEMS, Nanostructures [MMN] | |
hal.structure.identifier | Efficient runtime systems for parallel architectures [RUNTIME] | |
hal.structure.identifier | Agence Universitaire de la Francophonie [AUF] | |
dc.contributor.author | BRUNET, Edouard | |
hal.structure.identifier | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris [ESPCI Paris] | |
hal.structure.identifier | Department of Microbiology and National Key Laboratory for Agrobiotechnology [DEPARTMENT OF MICROBIOLOGY AND NATIONAL KEY LABORATORY FOR AGROBIOTECHNOLOGY] | |
hal.structure.identifier | School of Plant Biology [SPB] | |
hal.structure.identifier | AI-ECON Research Center | |
hal.structure.identifier | Center of Excellence for Document Analysis and Recognition [CEDAR] | |
hal.structure.identifier | Gilead Sciences | |
hal.structure.identifier | Hong Kong University of Science and Technology [HKUST] | |
dc.contributor.author | CHEN, Suelin | |
hal.structure.identifier | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris [ESPCI Paris] | |
dc.contributor.author | GOULPEAU, Jacques | |
hal.structure.identifier | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris [ESPCI Paris] | |
hal.structure.identifier | Neurobiologie et diversité cellulaire [NDC] | |
dc.contributor.author | LABAS, Valérie | |
hal.structure.identifier | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris [ESPCI Paris] | |
hal.structure.identifier | Neurobiologie et diversité cellulaire [NDC] | |
dc.contributor.author | VINH, Joelle | |
hal.structure.identifier | Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris [ESPCI Paris] | |
hal.structure.identifier | Laboratoire de Physique Statistique de l'ENS [LPS] | |
hal.structure.identifier | Laboratoire de Physico-Chimie Théorique [LPCT] | |
hal.structure.identifier | Laboratoire Microfluidique, MEMS, Nanostructures [MMN] | |
dc.contributor.author | TABELING, Patrick | |
dc.date.accessioned | 2024-04-15T09:56:17Z | |
dc.date.available | 2024-04-15T09:56:17Z | |
dc.date.issued | 2006-10 | |
dc.identifier.issn | 0003-2654 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/198820 | |
dc.description.abstractEn | A microfluidic poly(dimethylsiloxane) (PDMS) microdevice was realized, combining on-line protein electrophoretic separation, selection, and digestion of a protein of interest for identification by mass spectrometry. The system includes eight integrated valves and one micropump dedicated to control the flow operations. Myoglobin was successfully isolated from bovine serum albumin (BSA), then selected using integrated valves and digested in a rotary micromixer. Proteolytic peptides were recovered from the micromixer for protein identification. Total analysis from sample injection to protein identification is performed under 30 minutes, with samples of tens of nanolitres. The paper shows that PDMS technology can be successfully used for integrating complex preparation protocols of proteic samples prior to MS analysis. | |
dc.language.iso | en | |
dc.publisher | Royal Society of Chemistry | |
dc.title.en | PDMS-based microfluidics for proteomic analysis. | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1039/b606394b | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN] | |
bordeaux.journal | Analyst | |
bordeaux.page | 1122-8 | |
bordeaux.volume | 131 | |
bordeaux.hal.laboratories | Laboratoire Bordelais de Recherche en Informatique (LaBRI) - UMR 5800 | * |
bordeaux.issue | 10 | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | CNRS | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-00196106 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Non spécifiée | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-00196106v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analyst&rft.date=2006-10&rft.volume=131&rft.issue=10&rft.spage=1122-8&rft.epage=1122-8&rft.eissn=0003-2654&rft.issn=0003-2654&rft.au=DODGE,%20Arash&BRUNET,%20Edouard&CHEN,%20Suelin&GOULPEAU,%20Jacques&LABAS,%20Val%C3%A9rie&rft.genre=article |
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