Molecular cloning, sequence and expression of Aa-polB, a mitochondrial gene encoding a family B DNA polymerase from the edible basidiomycete Agrocybe aegerita. Mol Gen Genet
| dc.rights.license | open | en_US |
| dc.contributor.author | BOIS, F. | |
| hal.structure.identifier | Université de Bordeaux Ségalen [Bordeaux 2] | |
| dc.contributor.author | BARROSO, Gerard | |
| hal.structure.identifier | Environnements et Paléoenvironnements OCéaniques [EPOC] | |
| dc.contributor.author | GONZALEZ, Patrice
ORCID: 0000-0003-2628-2993 IDREF: 14341917X | |
| hal.structure.identifier | Université de Bordeaux Ségalen [Bordeaux 2] | |
| dc.contributor.author | LABARÈRE, Jacques | |
| dc.date.accessioned | 2024-04-12T07:38:37Z | |
| dc.date.available | 2024-04-12T07:38:37Z | |
| dc.date.issued | 1999-04-01 | |
| dc.identifier.issn | 0026-8925 | en_US |
| dc.identifier.uri | https://www.researchgate.net/publication/12975919_Molecular_cloning_sequence_and_expression_of_Aa-polB_a_mitochondrial_gene_encoding_a_family_B_DNA_polymerase_from_the_edible_basidiomycete_Agrocybe_aegerita._Mol_Gen_Genet | |
| dc.identifier.uri | oai:researchgate.net:12975919 | |
| dc.identifier.uri | oai:crossref.org:10.1007/s004380050994 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/197569 | |
| dc.description.abstractEn | An ORF of 1716 nucleotides, putatively encoding a DNA polymerase, was characterized in the mitochondrial genome of the edible basidiomycete Agrocybe aegerita. The complete gene, named Aa-polB, and its flanking regions were cloned and sequenced from three overlapping restriction fragments. Aa-polB is located between the SSU rDNA (5' region) and a gene for tRNA(Asn) (3' region), and is separated from these genes by two A + T-rich intergenic regions of 1048 (5' region) and 3864 (3' region) nucleotides, which lack repeated sequences of mitochondrial or plasmid origin. The deduced Aa-POLB protein shows extensive sequence similarity with the family B DNA polymerases encoded by genomes that rely on protein-primed replication (invertrons). The domains involved in the 3'-->5' exonuclease (Exo I to III) and polymerase (Pol I to Pol V) activities were localized on the basis of conserved sequence motifs. The alignment of the Aa-POLB protein (571 amino acids) with sequences of family B DNA polymerases from invertrons revealed that in Aa-POLB the N-terminal region preceding Exo I is short, suggesting a close relationship with the DNA polymerases of bacteriophages that have linear DNA. The Aa-polB gene was shown to be present in all wild strains examined, which were collected from a wide range of locations in Europe. As shown by RT-PCR, the Aa-polB gene is transcribed in the mitochondria, at a low but significant level. The likelihood of the coexistence of Aa-POLB and Pol gamma in the A. aegerita mitochondrion is discussed in the light of recent reports showing the conservation of the nucleus-encoded Pol gamma from yeast to human. | |
| dc.language.iso | EN | en_US |
| dc.source | researchgate | |
| dc.source | crossref | |
| dc.title.en | Molecular cloning, sequence and expression of Aa-polB, a mitochondrial gene encoding a family B DNA polymerase from the edible basidiomycete Agrocybe aegerita. Mol Gen Genet | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1007/s004380050994 | en_US |
| dc.subject.hal | Sciences de l'environnement | en_US |
| bordeaux.journal | Molecular and General Genetics MGG | en_US |
| bordeaux.page | 508-513 | en_US |
| bordeaux.volume | 261 | en_US |
| bordeaux.hal.laboratories | EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805 | en_US |
| bordeaux.issue | 3 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | dissemin | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | dissemin | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20and%20General%20Genetics%20MGG&rft.date=1999-04-01&rft.volume=261&rft.issue=3&rft.spage=508-513&rft.epage=508-513&rft.eissn=0026-8925&rft.issn=0026-8925&rft.au=BOIS,%20F.&BARROSO,%20Gerard&GONZALEZ,%20Patrice&LABAR%C3%88RE,%20Jacques&rft.genre=article |
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