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Cellulose biosaccharification by Irpex lacteus wood decay fungus
| hal.structure.identifier | National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України] | |
| dc.contributor.author | BOIKO, Sergiy | |
| hal.structure.identifier | Biodiversité, Gènes & Communautés [BioGeCo] | |
| hal.structure.identifier | National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України] | |
| dc.contributor.author | NETSVETOV, Maksym | |
| hal.structure.identifier | National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine [NASU / НАН України] | |
| dc.contributor.author | RADCHENKO, Vladimir | |
| dc.date.issued | 2023-05 | |
| dc.identifier.issn | 0717-3644 | |
| dc.description.abstractEn | Enzymatic hydrolysis is an environmentally friendly technology to produce sugars from pretreated biomass. Here, we show that the new Il-11 Irpex lacteus strain can synthesize cellulases in a high quantity. The peptone and filter paper contained in the medium significantly enhanced activity of endo-1,4-fi-D-glucanases (app. 50 IU/mL) and total cellulases (app. 9 IU/mL), whereas the medium with peptone and sodium carboxymethyl cellulose stimulated activity of exo-1,4-fi-D-glucanases (33 IU/mL). The expression of cellulases reached its maximum within 96-144 hours, and the optimum pH is 3,7. Thermal treatment at 30 & DEG;C for 60 minutes activated endo-1,4-fi-D-glucanases and total cellulases, while exo-1,4-fi-D-glucanases activity was enhanced following 40 & DEG;C treatment. In total, the cellulases complex (300 IU/g) saccharified untreated cellulose by 38 % in 48 hours. Concentrate with filter paper activity 100 IU/g is the more balanced enzyme-substrate ratio (2 %), which allows prolonging the saccharification process that will have a positive effect on the cost of the final product. | |
| dc.language.iso | en | |
| dc.publisher | Universidad del Bío-Bío | |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject.en | Cellulose | |
| dc.subject.en | endo-1 | |
| dc.subject.en | 4-β-D-glucanases | |
| dc.subject.en | exo-1 | |
| dc.subject.en | Irpex lacteus | |
| dc.subject.en | saccharification | |
| dc.subject.en | thermostability | |
| dc.title.en | Cellulose biosaccharification by Irpex lacteus wood decay fungus | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.4067/s0718-221x2023000100435 | |
| dc.subject.hal | Sciences de l'environnement | |
| bordeaux.journal | MADERAS: Ciencia y Tecnología | |
| bordeaux.volume | 25 | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-04513450 | |
| hal.version | 1 | |
| hal.popular | non | |
| hal.audience | Internationale | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-04513450v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=MADERAS:%20Ciencia%20y%20Tecnolog%C3%ADa&rft.date=2023-05&rft.volume=25&rft.eissn=0717-3644&rft.issn=0717-3644&rft.au=BOIKO,%20Sergiy&NETSVETOV,%20Maksym&RADCHENKO,%20Vladimir&rft.genre=article |
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