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Characterization of tomato-associated rhizobacteria recovered from various tomato-growing sites in Tunisia

hal.structure.identifierUniversité de Sousse
dc.contributor.authorOUHAIBI-BEN ABDELJALIL, Nada
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorVALLANCE, Jessica
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
hal.structure.identifierBIOVITIS (FRANCE)
dc.contributor.authorGERBORE, Jonathan
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorBRUEZ, Emilie
hal.structure.identifierUnité de Recherche Oenologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARTINS, Guilherme
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorREY, Patrice
hal.structure.identifierUniversité de Sousse
dc.contributor.authorDAAMI-REMADI, Mejda
dc.date.accessioned2024-04-08T12:32:42Z
dc.date.available2024-04-08T12:32:42Z
dc.date.issued2016
dc.identifier.issn2157-7471
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197155
dc.description.abstractEnIn the present study, a total of 200 rhizobacterial isolates were obtained from rhizosphere of healthy tomato plants grown in fields with a history of severe soilborne diseases and mainly crown and root rots. Screened their capacity to suppress in vitro growth of Sclerotinia sclerotiorum and Rhizoctonia solani, 69 and 57 isolates out of the 200 tested were shown able to inhibit significantly the mycelial growth of target pathogens by 11-62% relative to control. The 25 most effective isolates, leading to suppression of both fungi by more than 45% over control, were selected and subjected to morphological, biochemical, molecular, and metabolic characterizations. This collection of tomato-associated rhizobacteria exhibited a great morphological and biochemical diversity. Sequencing of 16S rRNA and rpoB genes led to the identification of four genera namely Bacillus, Chryseobacterium, Enterobacter, and Klebsiella. The most frequent species were B. amyloliquefaciens, B. thuringiensis, B. megaterium, B. subtilis, E. cloacae, C. jejuense, and K. pneumoniae. Screening for their plant growth-promoting properties, 20 isolates were shown able to produce siderophore, 18 had solubilized phosphate, and 19 were capable to synthesize indole-3- acetic acid (IAA). PCR amplification of lipopeptide biosynthetic genes revealed the presence of genes encoding fengycin A and bacillomycin D biosynthesis in 18 and 16 isolates, respectively. Metabolic characterization performed using Biolog™ Ecoplates indicated that tomato-associated rhizobacteria displayed a large metabolic activity and they were able to use a wide range of carbon sources with the increase of the incubation duration. Based on their metabolic profiles, these rhizobacterial isolates were grouped into eight major clusters generated at the different sampling times (24, 48 and 120 h of incubation). Average well-color development (AWCD) values were found to be positively correlated with the Shannon diversity index.
dc.language.isoen
dc.subjecttunisia
dc.subject.enantifungal activity
dc.subject.encharacterization
dc.subject.endiversity
dc.subject.enidentification
dc.subject.enrhizobacteria
dc.subject.entomato
dc.title.enCharacterization of tomato-associated rhizobacteria recovered from various tomato-growing sites in Tunisia
dc.typeArticle de revue
dc.identifier.doi10.4172/2157-7471.1000351
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
dc.subject.halSciences du Vivant [q-bio]/Ingénierie des aliments
dc.subject.halSciences de l'ingénieur [physics]/Génie des procédés
bordeaux.journalJournal of Plant Pathology and Microbiology
bordeaux.page12 p.
bordeaux.volume7
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.issue5
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02635168
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02635168v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Plant%20Pathology%20and%20Microbiology&rft.date=2016&rft.volume=7&rft.issue=5&rft.spage=12%20p.&rft.epage=12%20p.&rft.eissn=2157-7471&rft.issn=2157-7471&rft.au=OUHAIBI-BEN%20ABDELJALIL,%20Nada&VALLANCE,%20Jessica&GERBORE,%20Jonathan&BRUEZ,%20Emilie&MARTINS,%20Guilherme&rft.genre=article


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