Afficher la notice abrégée

Benzothiadiazole-primed defence responses and enhanced differential expression of defence genes in Vitis vinifera infected with biotrophic pathogens Erysiphe necator and Plasmopara viticola

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDUFOUR, Marie-Cecile
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorLAMBERT, C.
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorBOUSCAUT, Jerome
hal.structure.identifierUniversité Sciences et Technologies - Bordeaux 1 [UB]
dc.contributor.authorMÉRILLON, J.C.
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorCORIO-COSTET, Marie-France
dc.date.accessioned2024-04-08T12:31:36Z
dc.date.available2024-04-08T12:31:36Z
dc.date.issued2013
dc.identifier.issn0032-0862
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197086
dc.description.abstractEnBenzothiadiazole (BTH), a salicylic acid analogue, strengthens plant defence mechanisms against a broad spectrum of pathogens. The role of pre-treatment with BTH in enhancing resistance against infection with various isolates of downy and powdery mildews (Plasmopara viticola and Erysiphe necator) was investigated in grapevine leaves. Tools were developed to better assess the defence status of the plant. In compatible interactions amongst a set of 19 genes, more than 57·2% of differentiated transcripts from P. viticola infected-leaves (Pv-infected leaves) and 90% from E. necator-infected leaves (En-infected leaves) were down-regulated at 24 h post-inoculation (hpi), indicating a manipulation of host responses by the pathogens. BTH treatment enhanced grapevine defences, with pathogen growth inhibited by 61–98%, depending on the pathogen isolate. Treatment also triggered up-regulation of pathogenesis-related protein genes such as PR-1, PR-2, PR-3, PR-8 and PR-10 in Pv-infected leaves, and PR-3, PR-6 and PR-10 in En-infected leaves. Treatment with BTH also led to regulation of indole pathway transcripts; in particular, anthranilate synthase was down-regulated at 24 hpi in all infected leaves, then strongly up-regulated afterwards according to the rate of pathogen development. Quantitation of polyphenols and stilbenes showed that pterostilbene was specifically accumulated in pre-treated leaves and associated with biological efficacy and significant increases in PR protein gene transcripts. The temporal evolution of defence-related genes in pre-treated infected leaves suggests that grapevine responses vary depending on the inter- or intra-species variability of pathogens.
dc.language.isoen
dc.publisherWiley
dc.subject.enASM
dc.subject.enBTH
dc.subject.endowny and powdery mildew
dc.subject.engrapevine
dc.subject.enplant defence genes
dc.subject.enpolyphenols
dc.title.enBenzothiadiazole-primed defence responses and enhanced differential expression of defence genes in Vitis vinifera infected with biotrophic pathogens Erysiphe necator and Plasmopara viticola
dc.typeArticle de revue
dc.identifier.doi10.1111/j.1365-3059.2012.02628.x
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalPlant Pathology
bordeaux.page370-382
bordeaux.volume62
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.issue2
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02644301
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02644301v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Pathology&rft.date=2013&rft.volume=62&rft.issue=2&rft.spage=370-382&rft.epage=370-382&rft.eissn=0032-0862&rft.issn=0032-0862&rft.au=DUFOUR,%20Marie-Cecile&LAMBERT,%20C.&BOUSCAUT,%20Jerome&M%C3%89RILLON,%20J.C.&CORIO-COSTET,%20Marie-France&rft.genre=article


Fichier(s) constituant ce document

FichiersTailleFormatVue

Il n'y a pas de fichiers associés à ce document.

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée