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Over-expression of VvWRKY1 in grapevines induces expression of jasmonic acid pathway-related genes and confers higher tolerance to the downy mildew

hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorMARCHIVE, Chloé
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorLÉON, Céline
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorKAPPEL, Christian
hal.structure.identifierSucres & Échanges Végétaux-Environnement [Équipe du laboratoire EBI Poitiers] [SEVE]
dc.contributor.authorCOUTOS-THÉVENOT, Pierre
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorCORIO-COSTET, Marie-France
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorDELROT, Serge
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorLAUVERGEAT, Virginie
IDREF: 105631000
dc.date.accessioned2024-04-08T12:31:17Z
dc.date.available2024-04-08T12:31:17Z
dc.date.issued2013
dc.identifier.issn1932-6203
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/197064
dc.description.abstractEnMost WRKY transcription factors activate expression of defence genes in a salicylic acid- and/or jasmonic acid-dependent signalling pathway. We previously identified a WRKY gene, VvWRKY1, which is able to enhance tolerance to fungal pathogens when it is overexpressed in tobacco. The present work analyzes the effects of VvWRKY1 overexpression in grapevine. Microarray analysis showed that genes encoding defence-related proteins were up-regulated in the leaves of transgenic 35S::VvWRKY1 grapevines. Quantitative RT-PCR analysis confirmed that three genes putatively involved in jasmonic acid signalling pathway were overexpressed in the transgenic grapes. The ability of VvWRKY1 to trans-activate the promoters of these genes was demonstrated by transient expression in grape protoplasts. The resistance to the causal agent of downy mildew, Plasmopara viticola, was enhanced in the transgenic plants. These results show that VvWRKY1 can increase resistance of grapevine against the downy mildew through transcriptional reprogramming leading to activation of the jasmonic acid signalling pathway.
dc.language.isoen
dc.publisherPublic Library of Science
dc.title.enOver-expression of VvWRKY1 in grapevines induces expression of jasmonic acid pathway-related genes and confers higher tolerance to the downy mildew
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0054185
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences de l'environnement
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPLoS ONE
bordeaux.page8 p.
bordeaux.volume8
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.issue1
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.peerReviewedoui
hal.identifierhal-02647347
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02647347v1
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