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«BioMolChem», a tool to assess the defense status of grapevines after stimulations or not: from laboratory to the field

hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorCORIO-COSTET, Marie-France
hal.structure.identifierUnité Mixte de Recherche en Santé Végétale (INRA/ENITA) [UMRSV]
dc.contributor.authorCLUZET, Stéphanie
hal.structure.identifierUnité Mixte de Recherche en Santé Végétale (INRA/ENITA) [UMRSV]
dc.contributor.authorLAMBERT, Carole
hal.structure.identifierSanté et agroécologie du vignoble [UMR SAVE]
dc.contributor.authorDUFOUR, Marie-Cecile
dc.date.accessioned2024-04-08T12:30:06Z
dc.date.available2024-04-08T12:30:06Z
dc.date.issued2013
dc.date.conference2013-06-10
dc.identifier.isbn978-92-9067-267-8
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/196980
dc.description.abstractEnStimulating plant defenses is a promising alternative method for limiting pesticide use in agriculture. To assess the defense status of the grapevine we have developed a triple approach called "BioMolChem". - Biological tests measure the efficacy of grapevine defenses against two major biotrophic pathogens (Erysiphe necator, Plasmopara viticola). - Molecular assays by q-RT-PCR show the expression patterns (over-expression or repression) of 24 genes involved in grapevine defenses, and they can then be correlated or not with the level of protection, - BioChemical analyses of phenylpropanoides by HPLC are used to quantify and identify molecules of interest, and correlate them with specific gene expression (stilbene biosynthesis) and the acquired protection. This tool was tested on leaves after stimulation by different elicitors (acibenzolar-S-methyl, phosphonates), on grapevine: Cabernet Sauvignon cv., resistant genotypes to powdery and downy mildew, and in the vineyard. We obtained correlations between the expression of various genes and with the level of protection. Similarly, we found correlations between the presence of known and unknown polyphenols and the level of protection. Resveratrol, a well-known phytoalexin of the grapevine, is a good marker of defense status but not of protection. Therefore, we now have an valuable tool for understanding the defense and protection status of the grapevine in laboratory and field experiments.
dc.language.isoen
dc.publisherIOBC-WPRS
dc.title.en«BioMolChem», a tool to assess the defense status of grapevines after stimulations or not: from laboratory to the field
dc.typeAutre communication scientifique (congrès sans actes - poster - séminaire...)
dc.subject.halSciences du Vivant [q-bio]
bordeaux.page462 p.
bordeaux.volume89
bordeaux.hal.laboratoriesSanté et Agro-Ecologie du Vignoble (SAVE) - UMR 1065*
bordeaux.institutionBordeaux Sciences Agro
bordeaux.institutionINRAE
bordeaux.conference.titleWorking Group “Induced resistance in plants against insects and diseases”
bordeaux.countryFR
bordeaux.conference.cityAvignon
bordeaux.peerReviewedoui
hal.identifierhal-02744785
hal.version1
hal.invitednon
hal.conference.end2013-06-13
hal.popularnon
hal.audienceNationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02744785v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2013&rft.volume=89&rft.spage=462%20p.&rft.epage=462%20p.&rft.au=CORIO-COSTET,%20Marie-France&CLUZET,%20St%C3%A9phanie&LAMBERT,%20Carole&DUFOUR,%20Marie-Cecile&rft.isbn=978-92-9067-267-8&rft.genre=conference


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