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Design of thermoresponsive elastin-like glycopolypeptides for selective lectin binding and sorting
dc.rights.license | open | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | BRAVO ANAYA, Lourdes Monica M | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | PETITDEMANGE, Rosine | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | ROSSELIN, Marie | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | IBARBOURE, Emmanuel | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | GARBAY, Bertrand
IDREF: 033777551 | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | GARANGER, Elisabeth
IDREF: 089451740 | |
hal.structure.identifier | Department of Chemistry and Biochemistry [Los Angeles] | |
dc.contributor.author | DEMING, Timothy | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | LECOMMANDOUX, Sebastien | |
dc.date.accessioned | 2020 | |
dc.date.available | 2020 | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1525-7797 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/19671 | |
dc.description.abstractEn | Selective lectin binding and sorting was achieved using thermosensitive glycoconjugates derived from recombinant elastin-like polypeptides (ELPs) in simple centrifugation-precipitation assays. A recombinant ELP, (VPGXG)40, containing periodically spaced methionine residues was used to enable chemoselective post-synthetic modification via thioether alkylation using alkyne functional epoxide derivatives. The resulting sulfonium groups were selectively demethylated to give alkyne functionalized homocysteine residues, which were then reacted with azido-functionalized monosaccharides to obtain ELP glycoconjugates with periodic saccharide functionality. These modifications were also found to allow modulation of ELP temperature dependent water solubility. The multivalent ELP glycoconjugates were evaluated for specific recognition, binding and separation of the lectin Ricinus communis agglutinin (RCA120) from a complex protein mixture. RCA120 and ELP glycoconjugate interactions were evaluated using laser scanning confocal microscopy and dynamic light scattering. Due to the thermoresponsive nature of the ELP glycoconjugates, it was found that heating a mixture of galactose-functionalized ELP and RCA120 in complex media selectively yielded a phase separated pellet of ELP-RCA120 complexes. Based on these results, ELP glycoconjugates show promise as designer biopolymers for selective protein binding and sorting. | |
dc.description.sponsorship | Développement de squelettes polypeptidiques recombinants pour la synthèse de glycoconjugués multivalents parfaitement définis - ANR-15-CE07-0002 | |
dc.language.iso | en | |
dc.publisher | American Chemical Society | |
dc.subject.en | click chemistry | |
dc.subject.en | lectins | |
dc.subject.en | chemoselective post-modifications | |
dc.subject.en | Elastin-like polypeptides (ELPs) | |
dc.subject.en | saccharides | |
dc.subject.en | glycoconjugates | |
dc.title.en | Design of thermoresponsive elastin-like glycopolypeptides for selective lectin binding and sorting | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1021/acs.biomac.0c00374 | |
dc.subject.hal | Chimie/Polymères | |
dc.subject.hal | Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire | |
bordeaux.journal | Biomacromolecules | |
bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02618863 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02618863v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomacromolecules&rft.date=2020&rft.eissn=1525-7797&rft.issn=1525-7797&rft.au=BRAVO%20ANAYA,%20Lourdes%20Monica%20M&PETITDEMANGE,%20Rosine&ROSSELIN,%20Marie&IBARBOURE,%20Emmanuel&GARBAY,%20Bertrand&rft.genre=article |
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